Multi-layered polymerizing hydrogels for tissue regeneration
First Claim
1. A method of producing a multi-layered tissue construct comprising the steps of:
- providing a first polymerizable mixture, including, optionally, a first polymerization initiator;
providing a second polymerizable mixture, including, optionally, a second polymerization initiator;
wherein one of the first and second mixtures comprises a cells;
placing a volume of the first mixture in a space, then crosslinking the first mixture for a first predetermined time until the first mixture forms an at least partially gelled first layer; and
placing a volume of the second mixture in the space with the at least partially gelled first layer, then crosslinking the second mixture for a second predetermined time until the second mixture is at least partially gelled to form a second layer.
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Abstract
A multi-layered tissue construct includes: a first layer comprising a first hydrogel; and a second layer comprising a second hydrogel, wherein the first layer is connected to the second layer at a first transition zone and wherein at least one of the first layer and the second layer further comprises a component selected from the group consisting of cells and a bioactive substance. Another multi-layered tissue construct includes: a first layer comprising a first hydrogel; a second layer comprising cells of a first type, wherein the second layer is disposed on the first layer; and a third layer comprising a second hydrogel and optionally cells of the first type encapsulated in the second hydrogel, wherein the third layer is disposed on the second layer. Methods for producing these multi-layered tissue constructs are also disclosed.
89 Citations
52 Claims
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1. A method of producing a multi-layered tissue construct comprising the steps of:
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providing a first polymerizable mixture, including, optionally, a first polymerization initiator;
providing a second polymerizable mixture, including, optionally, a second polymerization initiator;
wherein one of the first and second mixtures comprises a cells;
placing a volume of the first mixture in a space, then crosslinking the first mixture for a first predetermined time until the first mixture forms an at least partially gelled first layer; and
placing a volume of the second mixture in the space with the at least partially gelled first layer, then crosslinking the second mixture for a second predetermined time until the second mixture is at least partially gelled to form a second layer. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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24. A multi-layered tissue construct comprising:
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a first layer comprising a first hydrogel; and
a second layer comprising a second hydrogel, wherein the first layer is connected to the second layer at a first transition zone and wherein at least one of the first layer and the second layer further comprises cells. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 35, 36, 37, 38)
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Specification