Bioassay method, bioassay device, and bioassay substrate

• US 20050069880A1
• Filed: 05/21/2003
• Published: 03/31/2005
• Est. Priority Date: 05/21/2002
• Status: Active Grant

• Current Assignee: Sony Corporation (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Yamamoto, Takuro, Mamine, Takayoshi
• Granted Patent: US 8,623,597 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 2219/0036 Nozzles
• CPC Class Codes: B01J 2219/00362 Acoustic nozzles
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00536 in the shape of disks
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

Disclosed is a bioassay method in which, by controlling the electric field formation in the reaction region where an interaction between substances, such as a hybridization, is performed, the efficiency of the interaction can be improved. Also disclosed is a bioassay apparatus in which the method can be favorably carried out. In the method, an interaction between substances is detected by a detecting element 1 (10), the detecting element including at least a detection surface S (S′) which is surface-treated for immobilizing a detecting substance D, a reaction region R (R′) which provides a field for interaction between the detecting substance D immobilized on the detection surface S (S′) and a target substance T, and an electric field-forming means E which forms an electric field in the reaction region R (R′) by applying a potential difference in the reaction region R (R′), and the method includes at least a step of turning on/off the electric field formation by the electric field-forming means E at a predetermined timing.

Claims

1. A bioassay method comprising detecting an interaction between substances by a detecting element, the detecting element comprising at least:

a detection surface which is surface-treated for immobilizing a detecting substance;

a reaction region which provides a field for interaction between the detecting substance immobilized on the detection surface and a target substance; and

an electric field-forming means which forms an electric field in the reaction region by applying a potential difference in the reaction region,

wherein the method comprises at least a step of turning on/off the electric field formation by the electric field-forming means at a predetermined timing.

2. A bioassay method according to claim 1, wherein the detecting substance and the target substance are nucleotide chains, and the interaction is a hybridization.

3. A bioassay method according to claim 2, further comprising the steps of:

forming an electric field so as to stretch the detecting nucleotide chain immobilized on the detection surface at the end;

subsequently adding the target nucleotide chain into the reaction region;

subsequently forming an electric field so as to relatively move the detecting nucleotide chain and the target nucleotide chain in the reaction region; and

subsequently performing the hybridization with the electric field being removed.

7. A bioassay substrate according to claim 3claim 6, wherein the cell-type detecting elements are arrayed radially, when viewed from above, on the disc-shaped substrate.

8. A bioassay substrate according to claim 7, wherein different detecting nucleotide chains are immobilized for each cell-type detecting element or for each group of cell-type detecting elements.

4. A bioassay apparatus comprising a detecting element, the detecting element comprising at least:

a detection surface which is surface-treated for immobilizing a detecting substance;

a reaction region which provides a field for interaction between the detecting substance immobilized on the detection surface and a target substance; and

an electric field-forming means which can form an electric field in the reaction region by applying a potential difference and which can turn on/off the electric field formation at a predetermined timing.

5. A bioassay substrate comprising a detecting element provided on a disc-shaped substrate from which recorded information can be optically read, the detecting element comprising at least:

(1) a detection surface which is surface-treated for immobilizing an end of a detecting nucleotide chain;

(2) positive and negative electrodes for forming an electric field to stretch the detecting nucleotide chain immobilized on the detection surface; and

(3) a reaction region which provides a field for hybridization between the detecting nucleotide chain and a target nucleotide chain.

6. A bioassay substrate according to claim 5, wherein the detecting element is a cell-type detecting element in which the detection surface is placed on one surface, and a plurality of cell-type detecting elements are arrayed on the disc-shaped substrate.

9. A bioassay substrate according to claim 5, wherein the reaction region of the detecting element is provided in each of the grooves radially extending on the disc-shaped substrate, and the detection surface is placed on an inner surface of the groove.

10. A bioassay substrate according to claim 9, wherein different detecting nucleotide chains are immobilized for each groove or for each group of grooves.

11. A bioassay substrate according to claim 5, further comprising a means for providing the positional information of the detection surface site and rotational synchronization information.

12. A bioassay substrate according to claim 11, wherein the means comprises a wobbled groove or address pits provided on the substrate.

13. A bioassay substrate according to claim 5, wherein the reaction region is filled with a material which can undergo a reversible phase change between gel and sol at between room temperature and the optimal temperature for the reaction.

14. A bioassay substrate according to claim 5, wherein the hybridization is detected by an intercalator.