Method for the detection and/or identification of the original animal species in animal matter contained in a sample
First Claim
1. A method for determining an original animal species in a sample liable to contain an ingredient obtained from at least said species, characterized in that:
- a) a nucleic acid fraction obtained from said sample is provided, b) at least one reagent specific for the animal species is provided, chosen from the group consisting of the reference sequences SEQ ID Nos 1 to 232, and Nos 242 to 261, the sequences complementary to each of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261, respectively, the complementarity meaning any sequence capable of hybridizing, at a temperature of between 20 and 70°
C., in saline solution at a concentration of approximately 0.5 to 1 M, with any one of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261, the sequences homologous to each of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261 and of the sequences complementary to each of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261, respectively, the homology meaning any sequence, for example fragment, comprising a series of at least 5 contiguous nucleotides included in any one of said sequences, and exhibiting at least 70% identity with said any sequence, c) the nucleic acid fraction and said reagent are brought into contact, and d) any signal or item of information resulting from the specific reaction between said reagent and the nucleic acid fraction, characterizing the presence in said sample of said original animal species, is determined by means of detection.
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Abstract
Disclosed is a method for detecting or identifying the original animal species in a sample likely to contain an ingredient obtained at least from said species. The inventive method is characterized by the following steps: a) a nuclear fraction is obtained from said sample; b) at least one reactant which is specific to the animal species is provided and selected among a group formed by: - the reference sequences SEQ ID numbers 1 to 232, 242 to 261; - the sequences complementing each of the sequences SED ID numbers 1 to 232, 242 to 261, respectively; - the sequences homologous to each of the sequences SEQ ID numbers 1 to 232, 242 to 261 and sequences complementing each of the sequences SED ID numbers 1 to 232, 242 to 261, respectively; c) the nuclear fraction and said reactant are reacted with each other; and d) any signal or information resulting from the specific reaction between said reactant and the nuclear fraction is detected, whereby it can be established if said sample contains said original animal species.
15 Citations
17 Claims
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1. A method for determining an original animal species in a sample liable to contain an ingredient obtained from at least said species, characterized in that:
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a) a nucleic acid fraction obtained from said sample is provided, b) at least one reagent specific for the animal species is provided, chosen from the group consisting of the reference sequences SEQ ID Nos 1 to 232, and Nos 242 to 261, the sequences complementary to each of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261, respectively, the complementarity meaning any sequence capable of hybridizing, at a temperature of between 20 and 70°
C., in saline solution at a concentration of approximately 0.5 to 1 M, with any one of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261,the sequences homologous to each of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261 and of the sequences complementary to each of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261, respectively, the homology meaning any sequence, for example fragment, comprising a series of at least 5 contiguous nucleotides included in any one of said sequences, and exhibiting at least 70% identity with said any sequence, c) the nucleic acid fraction and said reagent are brought into contact, and d) any signal or item of information resulting from the specific reaction between said reagent and the nucleic acid fraction, characterizing the presence in said sample of said original animal species, is determined by means of detection. - View Dependent Claims (2, 9)
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3. A nucleotide sequence characterized in that it is chosen from the group consisting of:
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a) the reference sequences SEQ ID Nos 1 to 232, and Nos 242 to 261, b) the sequences complementary to each of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261, respectively, the complementarity meaning any sequence capable of hybridizing, at a temperature of between 20 and 70°
C., in saline solution at a concentration of approximately 0.5 to 1 M, with any one of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261,c) the sequences homologous to each of the sequences SEQ ID Nos 1 to 232, and Nos 242 to 261, and of the sequences according to b), respectively, the homology meaning any sequence, for example fragment, comprising a series of at least 5 contiguous nucleotides included in any one of said sequences, and exhibiting at least 70% identity with said any sequence. - View Dependent Claims (4, 5, 6, 7, 8)
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10. A nucleotide sequence characterized in that it is chosen from the group consisting of:
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a) the reference sequences SEQ ID Nos 235 to 239, and 262 to 271, b) the sequences complementary to each of the sequences SEQ ID Nos 235 to 239, and 262 to 271, respectively, the complementarity meaning any sequence capable of hybridizing, at a temperature of between 20 and 70°
C., in saline solution at a concentration of approximately 0.5 to 1M, with any one of the sequences SEQ ID Nos 235 to 239, and 262 to 271,c) the sequences homologous to each of the sequences SEQ ID Nos 235 to 239, and 262 to 271, and of the sequences according to b), respectively, the homology meaning any sequence, for example fragment, comprising a series of at least 5 contiguous nucleotides included in any one of said sequences and also a group of two or three nucleotides belonging to a region which has been conserved for all the species of a group under consideration, and said sequence exhibiting at least 70% identity with said any sequence. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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Specification