Method and apparatus for classifying B-lymphocytes
First Claim
1. A method for classifying B-lymphocytes, comprising the steps of:
- (1) preparing a measurement sample by mixing a blood sample with a lysing reagent to lyze erythrocytes and to shrink B-lymphocytes in said blood sample;
(2) letting said measurement sample flow through a flow cell of a flow cytometer;
(3) radiating a light beam onto a cell in the measurement sample that is flowing through the flow cell;
(4) detecting at least two scattered light emitted from the irradiated cell;
(5) specifying a region of a B-lymphocyte cluster in accordance with the detected scattered light; and
(6) counting the number of B-lymphocytes in the specified region.
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Abstract
A B-lymphocyte classifying method includes the steps of (1) preparing a measurement sample by mixing a blood sample with a lysing reagent to lyze erythrocytes and to shrink the B-lymphocytes in the blood sample, (2) letting the measurement sample flow through a flow cell of a flow cytometer, (3) radiating a light beam onto a cell in the measurement sample that is flowing through the flow cell, (4) detecting at least two scattered light emitted from the irradiated cell, (5) specifying a region of a B-lymphocyte cluster in accordance with the detected scattered light, and (6) counting the number of B-lymphocytes in the specified region.
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Citations
14 Claims
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1. A method for classifying B-lymphocytes, comprising the steps of:
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(1) preparing a measurement sample by mixing a blood sample with a lysing reagent to lyze erythrocytes and to shrink B-lymphocytes in said blood sample;
(2) letting said measurement sample flow through a flow cell of a flow cytometer;
(3) radiating a light beam onto a cell in the measurement sample that is flowing through the flow cell;
(4) detecting at least two scattered light emitted from the irradiated cell;
(5) specifying a region of a B-lymphocyte cluster in accordance with the detected scattered light; and
(6) counting the number of B-lymphocytes in the specified region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. An apparatus for classifying B-lymphocytes, comprising:
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(1) a measurement sample preparing section for preparing a measurement sample by mixing a blood sample with a lysing reagent to lyze erythrocytes and to shrink B-lymphocytes in said blood sample;
(2) a flow cell for introducing said measurement sample;
(3) a light source for radiating a light beam onto a cell in the measurement sample that is flowing through the flow cell;
(4) a first scattered light detector and a second scattered light detector for detecting scattered light emitted from the irradiated cell;
(5) an analyzing section for specifying a region of a B-lymphocyte cluster in accordance with the detected scattered light and counting the number of B-lymphocytes in the specified region. - View Dependent Claims (10, 11, 12, 13, 14)
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Specification