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Method and apparatus for classifying B-lymphocytes

  • US 20050069959A1
  • Filed: 09/20/2004
  • Published: 03/31/2005
  • Est. Priority Date: 09/26/2003
  • Status: Active Grant
First Claim
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1. A method for classifying B-lymphocytes, comprising the steps of:

  • (1) preparing a measurement sample by mixing a blood sample with a lysing reagent to lyze erythrocytes and to shrink B-lymphocytes in said blood sample;

    (2) letting said measurement sample flow through a flow cell of a flow cytometer;

    (3) radiating a light beam onto a cell in the measurement sample that is flowing through the flow cell;

    (4) detecting at least two scattered light emitted from the irradiated cell;

    (5) specifying a region of a B-lymphocyte cluster in accordance with the detected scattered light; and

    (6) counting the number of B-lymphocytes in the specified region.

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