Tobacco having reduced nicotine and nitrosamines
First Claim
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1. A method of making a reduced nicotine tobacco plant comprising:
- identifying a tobacco plant in a field for nicotine reduction; and
contacting said tobacco plant with a composition selected from the group consisting of an auxin, auxin analog, and jasmonate antagonist from between about 21 days before topping to about 21 days after topping said tobacco plant, whereby the amount of nicotine in said topped tobacco plant contacted with said composition is below that of a topped tobacco plant of the same variety, grown under the same conditions, which has not been contacted with said composition.
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Abstract
Aspects of the present invention concern tobacco having reduced nicotine and nitrosamines, which is used to manufacture consumer tobacco products, and methods to produce such tobacco. More specifically, embodiments include methods to reduce nicotine and nitrosamines in tobacco crops by applying auxin, auxin analogs, and jasmonate antagonists during the cultivation of such tobacco crops. Tobacco generated using said methods and consumer tobacco products obtained therefrom are also embodiments.
204 Citations
25 Claims
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1. A method of making a reduced nicotine tobacco plant comprising:
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identifying a tobacco plant in a field for nicotine reduction; and
contacting said tobacco plant with a composition selected from the group consisting of an auxin, auxin analog, and jasmonate antagonist from between about 21 days before topping to about 21 days after topping said tobacco plant, whereby the amount of nicotine in said topped tobacco plant contacted with said composition is below that of a topped tobacco plant of the same variety, grown under the same conditions, which has not been contacted with said composition. - View Dependent Claims (2)
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3. A method of making cured, reduced nicotine tobacco comprising:
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identifying a tobacco plant in a field for nicotine reduction;
contacting said tobacco plant with a composition selected from the group consisting of an auxin, auxin analog, and jasmonate antagonist from between about 21 days before topping to about 21 days after topping said tobacco plant;
harvesting said tobacco plant; and
curing the tobacco of said harvested tobacco plant, whereby the amount of nicotine in said cured tobacco is below that of cured tobacco from a topped tobacco plant of the same variety, grown under the same conditions, which has not been contacted with said composition. - View Dependent Claims (4, 7, 9, 10, 12)
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5. A method of making a cured tobacco with a reduced amount of a tobacco specific nitrosamine (TSNA) comprising:
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identifying a tobacco plant in a field for nitrosamine reduction;
contacting said tobacco plant with a composition selected from the group consisting of an auxin, auxin analog, and jasmonate antagonist from between about 21 days before topping to about 21 days after topping said tobacco plant, whereby the amount of nicotine in said topped tobacco plant contacted with said composition is below that of a topped tobacco plant of the same variety, grown under the same conditions, which has not been contacted with said composition;
harvesting said topped tobacco plant that was contacted with said composition; and
curing the tobacco of said harvested tobacco plant, whereby the amount of a TSNA in said cured tobacco is below that of cured tobacco of a topped tobacco plant of the same variety, grown under the same conditions, which has not been contacted with said composition. - View Dependent Claims (6, 8, 11)
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13. A method of treating a tobacco plant to reduce the amount of nicotine comprising:
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contacting a tobacco plant of a first variety with at least one composition comprising an agent selected from the group consisting of an auxin, auxin analog, and jasmonate antagonist, wherein the contacting occurs from between about 21 days before topping to about 21 days after topping; and
obtaining a leaf of said tobacco plant, the leaf having a level of nicotine that is lower than a level of nicotine in a similar leaf of a topped plant of the same variety, cultivated under the same growing conditions, not contacted with the composition. - View Dependent Claims (14, 15, 16, 17, 18)
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- 19. A topped tobacco plant treated with an auxin or an auxin analog, wherein the amount of auxin in a leaf of said topped tobacco plant two days after topping is between about 5 and about 40 ng/g fresh weight.
Specification