Functionalized apertures for the detection of chemical and biological materials
First Claim
1. An apparatus comprising:
- a substrate having at least one aperture having a tapered portion with a top diameter greater than a bottom diameter and wherein the tapered portion of the aperture transitions into a cylindrical portion having a diameter equal to said bottom diameter of said tapered portion;
cross-linkers attached to the inner walls of said aperture; and
a macro-cyclic ring, having a diameter substantially the same as the diameter of the cylindrical portion of said aperture, attached at or near the circumference of one end of the cylindrical portion of said aperture.
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Accused Products
Abstract
Disclosed are nanometer to micron scale functionalized apertures constructed on a substrate made of glass, carbon, semiconductors or polymeric materials that allow for the real time detection of biological materials or chemical moieties. Many apertures can exist on one substrate allowing for the simultaneous detection of numerous chemical and biological molecules. One embodiment features a macrocyclic ring attached to cross-linkers, wherein the macrocyclic ring has a biological or chemical probe extending through the aperture. Another embodiment achieves functionalization by attaching chemical or biological anchors directly to the walls of the apertures via cross-linkers.
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Citations
11 Claims
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1. An apparatus comprising:
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a substrate having at least one aperture having a tapered portion with a top diameter greater than a bottom diameter and wherein the tapered portion of the aperture transitions into a cylindrical portion having a diameter equal to said bottom diameter of said tapered portion;
cross-linkers attached to the inner walls of said aperture; and
a macro-cyclic ring, having a diameter substantially the same as the diameter of the cylindrical portion of said aperture, attached at or near the circumference of one end of the cylindrical portion of said aperture. - View Dependent Claims (2, 3, 4, 5, 6)
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7. An apparatus comprising:
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a substrate having at least one aperture having a tapered portion with a top diameter greater than a bottom diameter and wherein the tapered portion of the aperture transitions into a cylindrical portion having a diameter equal to said bottom diameter of said tapered portion;
cross-linkers attached to the inner walls of said aperture; and
antibodies or chemical functional groups deposited around the inner walls of the aperture or around the circumference of one end of said aperture. - View Dependent Claims (8, 9)
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10. A method comprising:
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providing a substrate having at least one aperture having a tapered portion with a top diameter greater than the bottom diameter and wherein the tapered portion of the aperture transitions into a cylindrical portion having a diameter equal to said bottom diameter of said tapered portion; and
functionalizing said aperture to bind to a specific biological or chemical moiety.
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11. A method comprising:
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providing a substrate having at least one aperture having a tapered portion with a top diameter greater than the bottom diameter and wherein the tapered portion of the aperture transitions into a cylindrical portion having a diameter equal to said bottom diameter of said tapered portion, wherein said aperture is functionalized to bind to a specific biological or chemical moiety; and
passing a sample through said aperture while simultaneously measuring the variation in ionic current across the depth of said aperture.
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Specification