Detection of small nucleic acids
First Claim
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1. A method, comprising:
- a) hybridizing an interfering RNA target to at least one nucleic acid that contains sequence not complementary to said interfering RNA target to generate a detection structure; and
b) detecting said detection structure.
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Abstract
The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
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31 Claims
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1. A method, comprising:
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a) hybridizing an interfering RNA target to at least one nucleic acid that contains sequence not complementary to said interfering RNA target to generate a detection structure; and
b) detecting said detection structure. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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- 21. A kit comprising a nucleic acid configured for forming a detection structure when hybridized to an interfering RNA target sequence, wherein the nucleic acid comprises sequence that is not complementary to said interfering RNA target sequence.
Specification