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Method for non-invasilvely determining the relative levels of two biological substances

  • US 20050074810A1
  • Filed: 06/17/2002
  • Published: 04/07/2005
  • Est. Priority Date: 06/22/2001
  • Status: Active Grant
First Claim
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1. A method for non-invasively determining the relative levels of two substances present in a biological system, said method comprising contacting an anodal chamber and a cathodal chamber of an iontophoresis device comprising reversible electrodes with a biological system;

  • extracting by reverse iontophoresis charged and uncharged substances from said biological system, and collecting said charged and uncharged substances each independently into the anodal chamber or the cathodal chamber;

    analysing the collected amount of at least a first extracted substance and a second extracted substance;

    wherein said first and second substances are selected in such a way that the transport and/or transference number of the first substance is independent of the transport and/or transference number of the second substance;

    subsequently, determining the extraction ratio of the first substance to the second substance to determine their relative levels in the biological system.

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