Tissue culture vessel

• US 20050074873A1
• Filed: 08/19/2004
• Published: 04/07/2005
• Est. Priority Date: 09/09/2003
• Status: Abandoned Application

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Shanler, Michael S., Hughes, Kevin M.
• Application Number:
  US10/922,679
• Publication Number:
  US 20050074873A1
• Time in Patent Office: Days
• US Class Current: 435/304.300
• CPC Class Codes: C12M 23/08 Flask, bottle or test tube
• CPC Class Codes: C12M 23/24 Gas permeable parts
• CPC Class Codes: C12M 37/02 Filters

Abstract

A tissue culture vessel includes opposed top and bottom walls and sidewalls extending between the top and bottom walls. The top wall is formed with a septum aperture and a resealable septum is mounted in the septum aperture to permit access to interior regions of the tissue culture vessel by a medical device for accessing tissue grown in the vessel. The top wall may also include a membrane aperture and a membrane may be mounted in the membrane aperture for providing gas communication to interior portions of the tissue culture vessel.

Claims

1. A tissue culture vessel having a base with a bottom wall and a plurality of sidewalls extending up from said bottom wall, a cover extending across said sidewalls and opposed to said bottom wall, said cover being formed with at least one opening extending therethrough and a septum extending across said opening for permitting access to interior portions of said vessel by a medical device.

2. The tissue culture vessel of claim 1, wherein the septum is formed from a material that is resealable after access by the medical device.

3. The tissue culture vessel of claim 2, wherein said septum is formed with at least one slit extending at least partly through said septum.

4. The tissue culture vessel of claim 3, wherein said at least one split comprises two slits intersecting one another for defining a cross-cut in said septum.

5. The tissue culture vessel of claim 1, wherein said aperture in said cover is substantially circular.

6. The tissue culture vessel of claim 5, wherein said bottom wall of said base is formed with a trough substantially opposed to said aperture in said cover for collecting liquid media at a location below said septum.

7. The tissue culture vessel of claim 1, wherein said aperture is an elongate aperture.

8. The tissue culture vessel of claim 7, wherein said bottom wall of said base includes an elongate trough formed therein and substantially aligned with said elongate aperture in said cover for collecting liquid media in said trough at locations substantially aligned with said septum.

9. The tissue culture vessel of claim 7, wherein said septum includes at least one slit extending at least partly through said septum for facilitating access by a medical device.

10. The tissue culture vessel of claim 9, wherein said at least one slit comprises a plurality of pairs of intersecting slits at spaced apart locations along said septum.

11. The tissue culture vessel of claim 1, wherein said septum is secured in a cap and wherein said cap is mounted to said aperture in said cover.

12. The tissue culture vessel of claim 11, wherein one said sidewall of said base is formed with a hollow neck for pouring liquid media into or out of said vessel.

13. The tissue culture vessel of claim 12, further comprising a closure releasably mounted to said neck.

14. The tissue culture vessel of claim 1, wherein said cover further includes a membrane for providing gas communication between the interior of said vessel and ambient surroundings.

15. The tissue culture vessel of claim 14, wherein said aperture is a septum aperture and wherein said cover further comprises a membrane aperture, said membrane being mounted to said membrane aperture.

16. The tissue culture vessel of claim 1, further comprising a cap mounted to said aperture in said cover, said septum being mounted in a portion of said cap, a second portion of said cap being formed with a membrane for providing gas communication to interior portions of said tissue culture vessel.

17. A tissue culture vessel having a bottom wall, a top wall opposed to said bottom wall and a plurality of sidewalls extending between said top and bottom walls, a hollow neck formed at one of said sidewalls and providing communication to interior portions of said vessel for pouring a liquid media into or out of said vessel, a closure securely mounted to said neck for selectively closing said neck, said top wall of said tissue culture vessel having a septum aperture formed therethrough, a septum mounted in said septum aperture and having at least one slit extending at least partly therethrough to permit communication of a medical device with interior portions of said vessel, a membrane aperture formed through said top wall and a breathable membrane being mounted in said membrane aperture.

18. The tissue culture vessel of claim 17, wherein said bottom wall of said vessel is formed to define a trough substantially aligned with said septum aperture.

19. The tissue culture vessel of claim 18, wherein said septum aperture and said trough are elongated, and wherein said at least one slit is configured for receiving a plurality of medical devices.