Adipose-derived stem cells and lattices

• US 20050076396A1
• Filed: 08/29/2003
• Published: 04/07/2005
• Est. Priority Date: 03/10/1999
• Status: Abandoned Application

• Current Assignee: University of Pittsburgh of The Commonwealth System of Higher Education
• Original Assignee: University of Pittsburgh of The Commonwealth System of Higher Education
• Inventors: Llull, Ramon, Hedrick, Marc H., Katz, Adam J., Zhu, Min, Lorenz, Hermann Peter, Benhaim, Prosper, Futrell, J. William, Ashjian, Peter H., Zuk, Patricia
• Application Number:
  US10/651,564
• Publication Number:
  US 20050076396A1
• Time in Patent Office: Days
• US Class Current: 800/8
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C12N 2500/25 Insulin-transferrin; Insulin-transferrin-selenium
• CPC Class Codes: C12N 2500/38 Vitamins
• CPC Class Codes: C12N 2500/42 Organic phosphate, e.g. beta glycerophosphate
• CPC Class Codes: C12N 2501/01 Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
• CPC Class Codes: C12N 2501/15 Transforming growth factor beta (TGF-β)
• CPC Class Codes: C12N 2501/33 Insulin
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 2533/90 Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0607 Non-embryonic pluripotent stem cells, e.g. MASC induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12N 5/0647 Haematopoietic stem cells; Uncommitted or multipotent progenitors
• CPC Class Codes: C12N 5/0653 Adipocytes; Adipose tissue
• CPC Class Codes: C12N 5/0654 Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
• CPC Class Codes: C12N 5/0655 Chondrocytes; Cartilage
• CPC Class Codes: C12N 5/0658 Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
• CPC Class Codes: C12N 5/0667 Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
• CPC Class Codes: C12N 9/1276 RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
• CPC Class Codes: C12Y 207/07049 : RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Abstract

The present invention provides adipose-derived stem cells (ADSCs), adipose-derived stem cell-enriched fractions (ADSC-EF) and adipose-derived lattices, alone and combined with the ADSCs of the invention. In one aspect, the present invention provides an ADSC substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The ADSCs can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the ADSCs can be expanded and cultured to produce molecules such as hormones, and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides a adipose-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue. The lattice can be used as a substrate to facilitate the growth and differentiation of cells, whether in vivo or in vitro, into anlagen or even mature tissues or structures.

Claims

1. An isolated adipose-derived stem cell (ADSC).

2. The stem cell of claim 1, which can be cultured for at least 15 passages without differentiating.

3. The stem cell of claim 1, that is multipotent.

4. The stem cell of claim 3, that differentiates into mesoderm, ectoderm or endoderm.

6. The stem cell of claim 1 which is human.

7. The stem cell of claim 1, which is genetically modified.

8. A defined cell population comprising a plurality of the cell of claim 1.

9. The defined cell population of claim 8 which is homogenous.

10. The defined cell population of claim 8 which is heterogeneous.

11. The defined cell population of claim 8 which is clonal.

12. A progeny cell of the stem cell of claim 4, committed to develop into a mesodermal cell.

13. A progeny cell of the stem cell of claim 4, committed to develop into an ectodermal cell.

14. A progeny cell of the stem cell of claim 4, committed to develop into an endodermal cell.

15. Tissue comprised of the stem cell of claim 4, and differentiated mesodermal cells.

16. Tissue comprised of the stem cell of claim 4, and differentiated ectodermal cells.

17. Tissue comprised of the stem cell of claim 4, and differentiated into endodermal cells.

18. A method of inducing mesodermal tissue comprising culturing the stem cell of claim 4 in a mesoderm-inducing medium.

19. A method of inducing ectodermal tissue comprising culturing the stem cell of claim 4 in a ectoderm-inducing medium.

20. A method of inducing endodermal tissue comprising culturing the stem cell of claim 4 in a endoderm-inducing medium.

5. An adipose-derived stem-cell enriched fraction (ADSC-EF) of an adipose tissue sample from a subject, said fraction substantially free of adipocytes.

21-43. -43. (canceled)