In vivo bioreactors
First Claim
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1. A method for promoting generation of soft tissue, or precursor cells for soft tissue, comprising the steps of:
- i. creating an artificial space or environment in an organ or cavity of an animal; and
ii. introducing into the artificial space a matrix which is conducive to infiltration by, and growth and/or differentiation of pluripotent cells from the tissue surrounding the artificial space.
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Abstract
The present invention relates to an in vivo method of promoting the growth of autologous tissue and its use to form corrective structures, including tissue that can be explanted to other locations in the animal. In particular, the invention relates to methods and systems for (a) the site-specific regeneration of tissue, and (b) the synthesis of neotissue for transplantation.
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Citations
33 Claims
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1. A method for promoting generation of soft tissue, or precursor cells for soft tissue, comprising the steps of:
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i. creating an artificial space or environment in an organ or cavity of an animal; and
ii. introducing into the artificial space a matrix which is conducive to infiltration by, and growth and/or differentiation of pluripotent cells from the tissue surrounding the artificial space. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 32, 33)
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22. A method for promoting generation of cartilage or bone tissue, comprising the steps of:
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i. creating an artificial space in or adjacent periosteum tissue of an animal; and
ii. introducing into the artificial space a porous, biodegradable polymer matrix which is compatible with growth of chondrocytes from the periosteum surrounding the artificial space. - View Dependent Claims (23, 24, 25, 26, 27, 28)
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29. A kit for promoting generation of tissue in vivo, comprising:
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a. a tissue retractor for generating the artificial space;
b. a matrix which is conducive to infiltration by, and growth and/or differentiation of pluripotent cells; and
c. (optionally) an agent to partially degrade the connective tissue at the site, freeing cells to promote formation of the space and/or promote migration of cells into the space. - View Dependent Claims (31)
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30. A kit for promoting generation of tissue in vivo, comprising:
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a. a matrix precursor(s) capable of forming a dimensionally stable matrix in vivo, which matrix is conducive to infiltration by, and growth and/or differentiation of pluripotent cells, which matrix precursor(s) is in the form of a solution;
b. a TGF-β and
b-FGF, admixed with said matrix precursor(s) or in a form which is amenable to mixture with said matrix precursor(s); and
c. instructions (written or pictorial) associated with said kit, said instructions describing the preparation of the matrix precursor(s), TGF-β and
b-FGF for injection into an artificial space or environment in vivo.
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Specification