Device and method for manufacturing bead array, and method for detecting target substance

• US 20050079592A1
• Filed: 08/31/2004
• Published: 04/14/2005
• Est. Priority Date: 09/01/2003
• Status: Active Grant

• Current Assignee: Seiko Epson Corporation (Seiko Group)
• Original Assignee: Seiko Epson Corporation (Seiko Group)
• Inventors: Takagi, Fumio
• Granted Patent: US 7,253,006 B2
• Time in Patent Office: Days
• US Class Current: 435/174
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00466 in a slurry
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00545 Colours
• CPC Class Codes: B01J 2219/00554 Physical means
• CPC Class Codes: B01J 2219/00563 Magnetic means
• CPC Class Codes: B01J 2219/00574 radioactive
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A device for manufacturing a bead array is provided including a container, a liquid including a bead having a biological substance fixed on a surface of the bead, and means for ejecting the liquid including the bead on a desired position on a solid-phase base. A method for manufacturing a bead array is also provided including: containing the liquid including the bead in a container; selectively ejecting the liquid on a desired position on the solid-phase base; and forming a reactive region where the liquid is ejected.

Claims

1. A device for manufacturing a bead array, comprising:

a container containing a liquid including a bead, the bead having a biological substance fixed on a surface thereof; and

an actuator selectively ejecting the liquid including the bead to a desired position on a solid-phase base.

2. The device for manufacturing a bead array according to claim 1, wherein the actuator further comprises an ink jetting actuator.

3. The device for manufacturing a bead array according to claim 1, wherein the container is adapted to separately contain at least two types of liquids including a bead.

4. A method for manufacturing a bead array, comprising:

storing a liquid including a bead in a container, the bead having a biological substance fixed on a surface thereof;

ejecting the liquid including the bead to a desired position on a solid-phase base; and

forming a reactive region on the solid-phase base where the liquid including the bead is ejected.

5. The method for manufacturing a bead array according to claim 4, wherein the reactive region includes a plurality of beads, each of the beads having a single type of biological substance fixed thereto.

6. The method for manufacturing a bead array according to claim 4, wherein the reactive region includes a plurality of bead groups, each bead group having a single type of biological substance fixed to each bead of the group.

7. The method for manufacturing a bead array according to claim 4, further comprising:

separately storing another liquid including a bead in the container, the bead of the another liquid having the biological substance fixed on a surface thereof and including an identifier; and

ejecting the another liquid on the solid-phase base.

8. The method for manufacturing a bead array according to claim 7, wherein the identifier comprises at least one of bead size, bead density, bead coloring, a fluorescent marker, a magnetic marker, and a radiation marker.

9. The method for manufacturing a bead array according to claim 4, wherein the biological substance comprises any one of nucleic acid and protein.

10. The method for manufacturing a bead array according to claim 4, wherein a diameter of the bead is from about 0.02 to about 10 μ

m inclusive.

11. The method for manufacturing a bead array according to claim 4, wherein a diameter of the bead is from about 0.2 to about 5 μ

m inclusive.

12. The method for manufacturing a bead array according to claim 4, wherein the solid-phase base comprises a microtiter plate.

13. The method for manufacturing a bead array according to claim 4, wherein the reactive region comprises:

a well on a base surface; and

a cover plate joined to the base surface while leaving a gap therebetween that is smaller than a diameter of the bead.

14. The method for manufacturing a bead array according to claim 13, wherein the solid-phase base and the cover plate comprise glass.