Absorbance monitoring
First Claim
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1. A method for monitoring the absorbance of a sample, which method includes:
- directing a first beam of light having a first wavelength along an optical path through a portion of the sample and measuring the absorbance of the first wavelength so as to obtain a reference absorbance;
directing a second beam of light having a second wavelength substantially along the optical path and measuring the absorbance of the sample so as to obtain a measured sample absorbance; and
analysing the reference absorbance and the measured sample absorbance to obtain a true sample absorbance.
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Abstract
A method for monitoring the absorbance of a sample, which method includes: directing a first beam of light having a first wavelength along an optical path through a portion of the sample and measuring the absorbance of the first wavelength so as to obtain a reference absorbance; directing a second beam of light having a second wavelength substantially along the optical path and measuring the absorbance of the sample so as to obtain a measured sample absorbance; and analysing the reference absorbance and the measured sample absorbance to obtain a true sample absorbance.
22 Citations
35 Claims
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1. A method for monitoring the absorbance of a sample, which method includes:
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directing a first beam of light having a first wavelength along an optical path through a portion of the sample and measuring the absorbance of the first wavelength so as to obtain a reference absorbance;
directing a second beam of light having a second wavelength substantially along the optical path and measuring the absorbance of the sample so as to obtain a measured sample absorbance; and
analysing the reference absorbance and the measured sample absorbance to obtain a true sample absorbance. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of monitoring the presence of an analyte on a surface, which method includes:
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permitting the surface to contact a reagent so that analyte present on the surface may (chemically) react with the reagent if the analyte is present;
directing a first wavelength along an optical path through a portion of the reagent and measuring the absorbance of the sample so as to obtain a reference absorbance;
directing a second beam of light having a second wavelength substantially along the optical path and measuring the absorbance so as to obtain a measured sample absorbance; and
analysing the reference absorbance and the measured sample absorbance to obtain a true sample absorbance which provides an indication the amount of analyte present on the sample which has reacted with the reagent. - View Dependent Claims (16, 17, 18, 19, 20, 21)
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22. A method of monitoring the absorbance of a sample, which method includes directing a first beam of infra red light along a path length through the sample and measuring the absorbance so as to obtain a reference absorbance;
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directing a second beam of green light along the path length through the sample and measuring the absorbance of the second beam in the sample so as to obtain a measured sample absorbance; and
analysing the reference absorbance and the measured sample absorbance so as to obtain a true sample absorbance. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35)
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23. Apparatus for monitoring the absorbance of a sample, which apparatus includes:
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a first light source arranged to emit light at a first wavelength through the sample along an optical path;
a second light source arranged to emit light at a second wavelength through the sample substantially along the same optical path; and
a detector arranged to measure the absorbance of the light emitted from the first light source and the second light source which has passed through the sample. - View Dependent Claims (24, 25, 26)
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Specification