Method for transferring a substance to or from a closed system
First Claim
1. A method for removing a fluid substance from a closed system comprising a penetrable cap in sealing engagement with an open end of a fluid-holding vessel, the method comprising the steps of:
- a) entering an interior space defined by the cap and vessel of the system, wherein the step of entering the interior space of the system comprises;
i) penetrating a first frangible seal affixed to the cap with a fluid transfer device such that air passageways are formed between the fluid transfer device and the first frangible seal; and
ii) penetrating a second frangible seal affixed to the cap and axially aligned below the first frangible seal with the fluid transfer device such that air passageways are formed between the fluid transfer device and the second frangible seal, thereby permitting air to be vented from the interior space of the system;
b) drawing at least a portion of the fluid substance contained in the vessel into the fluid transfer device; and
c) removing the fluid transfer device from the system.
6 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to a cap which can form an essentially leak-proof seal with an open-ended vessel capable of receiving and holding fluid specimens or other materials for analysis. To minimize potentially contaminating contact between a fluid sample present in the vessel and humans or the environment, the present invention features a cap having a frangible seal which is penetrable by a plastic pipette tip or other fluid transfer device. The cap further includes filtering means for limiting dissemination of an aerosol or bubbles once the frangible seal has been pierced. The filtering means is positioned between the frangible seal and retaining means. The retaining means is positioned on the cap above the filtering means and may be used to contain the filtering means within the cap. The retaining means may be comprised of a material which is penetrable by a fluid transfer device.
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Citations
26 Claims
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1. A method for removing a fluid substance from a closed system comprising a penetrable cap in sealing engagement with an open end of a fluid-holding vessel, the method comprising the steps of:
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a) entering an interior space defined by the cap and vessel of the system, wherein the step of entering the interior space of the system comprises;
i) penetrating a first frangible seal affixed to the cap with a fluid transfer device such that air passageways are formed between the fluid transfer device and the first frangible seal; and
ii) penetrating a second frangible seal affixed to the cap and axially aligned below the first frangible seal with the fluid transfer device such that air passageways are formed between the fluid transfer device and the second frangible seal, thereby permitting air to be vented from the interior space of the system;
b) drawing at least a portion of the fluid substance contained in the vessel into the fluid transfer device; and
c) removing the fluid transfer device from the system. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 24, 25, 26)
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16. A method for depositing a substance in a closed system comprising a penetrable cap in sealing engagement with an open end of a substance-holding vessel, the method comprising the steps of:
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a) entering an interior space defined by the cap and vessel of the system, wherein the step of entering the interior space of the system comprises;
i) penetrating a first frangible seal affixed to the cap with a substance transfer device; and
ii) penetrating a second frangible seal affixed to the cap and axially aligned below the first frangible seal with the substance transfer device;
b) depositing one or more substances in the vessel; and
c) removing the substance transfer device from the system. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23)
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Specification