Fluidic circuits for sample preparation including bio-discs and methods relating thereto

• US 20050084422A1
• Filed: 07/26/2004
• Published: 04/21/2005
• Est. Priority Date: 06/19/2003
• Status: Active Grant

• Current Assignee: Vindur Technologies, Inc.
• Original Assignee: Vindur Technologies, Inc.
• Inventors: Norton, James R., Kido, Horacio, Coombs, James H.
• Granted Patent: US 7,390,464 B2
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 2400/0694 vents used to stop and induce flow, backpressure valves
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor

Abstract

A fluidic circuit for receiving a fluid and separating a component of a fluid from the fluid comprises a separation chamber for receiving the fluid, an air chamber in fluid communication with the separation chamber, and a return channel in fluid communication with the separation chamber. In an advantageous embodiment, the fluidic circuit is subjected to a force, such as a centrifugal force, so that substantially all of the component of the fluid is moved to the return channel while substantially all remaining portions of the fluid are moved to the separation chamber.

Claims

1. A fluidic circuit for processing fluid, comprising:

a sample loading chamber for receiving an amount of fluid for processing, said sample loading chamber having a sample inlet port;

a sample pass through channel having a first end and a second end, said first end of said sample pass through channel in fluid communication with said sample loading chamber;

a separation chamber in fluid communication with said second end of said sample pass through channel;

a sample flow channel having a first and a second end, said first end of said sample flow channel in fluid communication with said sample pass through channel; and

an analysis chamber in fluid communication with said second end of said sample flow channel.

2. A fluidic circuit for processing fluid, comprising:

a sample loading chamber for receiving an amount of fluid for processing, said sample loading chamber including a sample inlet port;

a sample pass through channel having a first end and a second end, said first end of said sample pass through channel in fluid communication with said sample loading chamber;

a separation chamber in fluid communication with said second end of said sample pass through channel;

a sample flow channel having a first and a second end, said first end of said sample flow channel in fluid communication with said sample pass through channel;

a mixing chamber having a first end and a second end, said first end of said mixing chamber in fluid communication with said second end of said sample flow channel; and

an analysis chamber in fluid communication with said second end of said mixing chamber.

3. The fluidic circuit according to claim 2 further comprising:

a vent channel having a fist end and a second end, said first and of said vent channel in fluid communication with said analysis chamber; and

a vent port in fluid communication with said second end of said vent channel.

4. The fluidic circuit according to claim 3 further comprising:

a buffer loading chamber for receiving an amount of fluid, said buffer loading chamber including a buffer inlet port;

a buffer pass through channel having a first end and a second end, said first end of said buffer pass through channel in fluid communication with said buffer loading chamber; and

a buffer flow channel having a first and a second end, said first end of said sample flow channel in fluid communication with said second end of said buffer pass through channel, said second end of said buffer flow channel in fluid communication with said first end of said mixing chamber.

5. The fluidic circuit according to claim 4 further comprising:

a sample waste channel having a first end and a second end, said first end of said sample waste channel connected to and in fluid communication with said sample pass through channel;

a sample waste chamber in fluid communication with said second end of said sample waste channel;

a sample waste vent channel in fluid communication with said sample waste chamber; and

a sample vent port in fluid communication with said sample vent channel.

6. The fluidic circuit according to claim 4 further comprising:

a buffer waste channel having a first end and a second end, said first end of said buffer waste channel connected to and in fluid communication with said buffer pass through channel;

a buffer waste chamber in fluid communication with said second end of said buffer waste channel; and

a buffer waste vent channel in fluid communication with said buffer waste chamber; and

a buffer vent port in fluid communication with said buffer vent channel.

7. The fluidic circuit according to claim 4 further comprising:

a sample waste vent channel in fluid communication with said separation chamber; and

a sample vent port in fluid communication with said sample waste vent channel.

8. The fluidic circuit according to claim 7 further comprising a first capillary valve within said sample pass through channel.

9. The fluidic circuit according to claim 7 further comprising a second capillary valve at the junction of said second end of said sample flow channel and first end of said mixing chamber.

10. The fluidic circuit according to claim 7 further comprising a third capillary valve within said buffer pass through channel.