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Immunoassay element

  • US 20050084953A1
  • Filed: 10/07/2004
  • Published: 04/21/2005
  • Est. Priority Date: 06/13/1997
  • Status: Abandoned Application
First Claim
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1. An immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity caused by any of a reaction between the antigen and an enzyme-labelled antibody;

  • 2) a reaction between the antigen, an antibody and an enzyme-labelled antigen; and

    3) a reaction between the antigen, an enzyme-labelled antibody and a conjugate of the antigen with a high molecular weight compound;

    wherein said element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of said labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting said diffusible material into a lower molecular weight product;

    characterized in that said non-diffusible substrate is a substrate which reacts solely with said labelling enzyme and avoids reacting with said fragmenting enzyme.

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