Stem cell maturation for all tissue lines

• US 20050090004A1
• Filed: 06/08/2004
• Published: 04/28/2005
• Est. Priority Date: 01/16/2003
• Status: Abandoned Application

• Current Assignee: PrimeGen Biotech LLC
• Original Assignee: PrimeGen Biotech LLC
• Inventors: Sayre, Chauncey Bigelow
• Application Number:
  US10/864,788
• Publication Number:
  US 20050090004A1
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C12N 2501/235 Leukemia inhibitory factor [LIF]
• CPC Class Codes: C12N 2509/00 Methods for the dissociation of cells, e.g. specific use of enzymes
• CPC Class Codes: C12N 2517/04 Cells produced using nuclear transfer
• CPC Class Codes: C12N 5/061 Sperm cells, spermatogonia
• CPC Class Codes: C12N 5/0611 Primordial germ cells, e.g. embryonic germ cells [EG]

Abstract

The present invention provides a hybrid stem comprising an enucleated adult stem cell having a nucleus derived from a primordial sex cell or an embryonic stem cell. The primordial sex cell may be a spermatogonia or an oogonia from a donor animal or mammal. The enucleated adult stem cell may be fused to a primordial sex cell or an embryonic stem cell by many methods including but not limited to electrofusion, virus-based fusion methodology, chemical fusion or mechanical-based fusion.

Claims

15. A therapeutic composition comprising an enucleated adult stem cell having a nucleus from a primordial sex cell or an embryonic stem cell.

16. The therapeutic composition of claim 15 wherein the therapeutic composition is used to regenerate diseased or damaged tissues of an animal in need thereof.

17. The therapeutic composition of claim 15 wherein the diseased tissue is heart tissue.

18. The therapeutic composition of claim 15 wherein the diseased tissue is lung tissue.

19. A fused stem cell comprising:

an enucleated adult stem cell; and

one of a primordial sex cell or embryonic stem cell fused to the enucleated adult stem cell.

20. The fused stem cell comprising of claim 19 wherein the primordial sex cell is fused to the enucleated adult stem cell.

21. The fused stem cell of claim 20 wherein the enucleated adult stem cell is fused with the primordial sex cell using electrofusion.

22. The fused stem cell of claim 20 wherein the enucleated adult stem cell is fused with the primordial sex cell by a virus-based fusion methodology.

23. The fused stem cell of claim 20 wherein the enucleated adult stem cell is fused with the primordial sex using chemical fusion.

24. The HSC of claim 20 wherein the enucleated adult stem cell is fused with the primordial sex cell using mechanical-based fusion.

26. A hybrid stem cell (HSC) comprising:

an enucleated adult stem cell having a nucleus from a primordial sex cell.

1. A hybrid stem cell (HSC) comprising:

an enucleated adult stem cell having a nucleus from a primordial sex cell or an embryonic stem cell.

2. The HSC of claim 1 wherein the enucleated adult stem cell and primordial sex cell are derived from the same animal.

3. The HSC of claim 2 wherein the animal is a mammal.

4. The HSC of claim 1 wherein the enucleated adult stem cell and the embryonic stem cell are derived from the same animal.

5. The HSC of claim 1 wherein the enucleated adult stem cell has a nucleus from a primordial sex cell.

6. The HSC of claim 5 wherein the primordial sex cell is a spermatogonium cell.

7. The HSC of claim 5 wherein the primordial sex cell is an undifferentiated spermatogonium cell.

8. The HSC of claim 5 wherein the primordial sex cell is a differentiated spermatogonium cell.

9. The HSC of claim 5 wherein the primordial sex cell is an oogonium cell.

10. The HSC of claim 1 wherein the enucleated adult stem cell is fused with the primordial sex cell using electrofusion.

11. The HSC of claim 1 wherein the enucleated adult stem cell is fused with the primordial sex cell by a virus-based fusion methodology.

12. The HSC of claim 1 wherein the enucleated adult stem cell is fused with the primordial sex using chemical fusion.

13. The HSC of claim 1 wherein the enucleated adult stem cell is fused with the primordial sex cell using mechanical-based fusion.

14. The HSC of claim 1 wherein the HSC is biologically active in a post natal animal

25. The fused stem cell of claim 29 wherein the embryonic stem cell is fused to the enucleated adult stem cell.

27. The HSC of claim 26 wherein the enucleated adult stem cell and primordial sex cell are derived from the same animal.

28. The HSC of claim 27 wherein the animal is a mammal.

29. The HSC of claim 26 wherein the primordial sex cell is a spermatogonium cell.

30. The HSC of claim 26 wherein the primordial sex cell is an undifferentiated spermatogonium cell.

31. The HSC of claim 26 wherein the primordial sex cell is a differentiated spermatogonium cell.

32. The HSC of claim 26 wherein the enucleated adult stem cell and primordial sex cell are derived from different individuals within the same species.

33. The HSC of claim 26 wherein the primordial sex cell is an oogonium cell A hybrid stem cell (HSC) comprising:

32-1. The HSC of claim 26 wherein the enucleated adult stem cell and primordial sex cell are derived from the same individual.

34. An enucleated adult stem cell having a nucleus from an embryonic stem cell.

35. The HSC of claim 34 wherein the enucleated adult stem cell and embryonic stem cell are from the same species.

36. The HSC of claim 34 wherein the animal is a mammal.

37. HSC of claim 34 wherein the enucleated adult stem cell and the embryonic stem cell are derived from the same individual.