Small molecule printing
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Accused Products
Abstract
The present invention provides compositions and methods to facilitate the identification of compounds that are capable of interacting with a biological macromolecule of interest. In one aspect, a composition is provided that comprises an array of one or more types of chemical compounds attached to a solid support, wherein the density of the array of compounds is at least 1000 spots per cm2. In particularly preferred embodiments, these compounds are attached to the solid support through a covalent interaction. In general, these inventive arrays are generated by: (1) providing a solid support, wherein said solid support is functionalized with a selected chemical moiety capable of interacting with a desired chemical compound to form an attachment; (2) providing one or more solutions of one or more types of compounds to be attached to the solid support; and (3) delivering said one or more types of compounds to the solid support, whereby an array is formed and the array of compounds has a density of at least 1000 spots per cm2. In another aspect, the present invention provides methods for utilizing these arrays to identify small molecule partners for biological macromolecules of interest comprising: (1) providing an array of compounds, wherein the array has a density of at least 1000 spots per cm2; (2) contacting the array with one of more types of biological macromolecules of interest; and (3) determining the interaction of specific small molecule-biological macromolecule partners.
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Citations
40 Claims
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1-18. -18. (canceled)
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19. A method for forming an array of more than one type of non-oligomeric compound attached to a solid support the method comprising:
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providing a solid support, wherein said solid support is functionalized with a selected chemical moiety capable of interacting with more than one type of non-oligomeric chemical compound to form a covalent linkage;
providing one or more solutions of more than one type of non-oligomeric chemical compounds to be attached to the solid support; and
delivering said one or more solutions of said more than one type of chemical compounds to the solid support, whereby each of said chemical compounds is attached to the solid support through a covalent linkage, and whereby said array of compounds has a density of at least 1000 spots per cm2. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 36, 37, 38, 39, 40)
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27. A method for forming an array of one or more types of non-oligomeric chemical compounds attached to a solid support, the method comprising:
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providing a glass or polymeric solid support, wherein said solid support is functionalized with a selected chemical moiety capable of interacting with one or more types of non-oligomeric chemical compounds to form a covalent linkage;
providing one or more solutions of said one or more types of non-oligomeric compounds to be attached to the glass or polymer solid support; and
delivering said one or more solutions of said one or more types of non-oligomeric chemical compounds to the solid support, whereby each of said chemical compounds is attached to the solid support though a covalent linkages, and whereby said array of non-oligomeric compounds has a density of at least 1000 spots per cm2. - View Dependent Claims (28, 29, 30, 31, 32, 33)
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34. (canceled)
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35. (canceled)
Specification