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Embryonic or stem-like cell lines produced by cross species nuclear transplantation

  • US 20050095704A1
  • Filed: 08/25/2004
  • Published: 05/05/2005
  • Est. Priority Date: 08/19/1996
  • Status: Abandoned Application
First Claim
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1. A method of producing embryonic or stem-like cells comprising the following steps:

  • (i) inserting a desired human or mammalian cell or cell nucleus into an enucleated animal oocyte, wherein such oocyte is derived from a different animal species than the human or mammalian cell under conditions suitable for the formation of a nuclear transfer (NT) unit;

    (ii) activating the resultant nuclear transfer units;

    (iii) culturing said activated nuclear transfer units until greater than the 2-cell developmental stage; and

    (iv) culturing cells obtained from said cultured NT units to obtain embryonic or stem-like cells.

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