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Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells

  • US 20050095706A1
  • Filed: 08/27/2004
  • Published: 05/05/2005
  • Est. Priority Date: 10/03/2001
  • Status: Active Grant
First Claim
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1. A method of creating a population of cells comprising a synchronous population of cells that are cultured from embryonic stem cells, are characterized by an early rosette morphology, and are Sox1

  • , Pax6+, comprising the steps of;

    (a) propagating embryonic stem cells into embryoid bodies and (b) propagating embryoid bodies into a synchronous population of neural stem cells in the form of early rosettes, wherein this propagation is in the presence of FGF2, FGF8, FGF9 or FGF4.

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