Transgenic ungulates having reduced prion protein activity and uses thereof
First Claim
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1. A bovine comprising a non-naturally occurring mutation in one or both alleles of an endogenous prion nucleic acid
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Abstract
The invention provides cloned transgenic ungulates (e.g., bovines) in which prion protein activity is reduced by one or more genetically engineered mutations. Desirably, these transgenic bovines are also genetically modified to express xenogenous (e.g., human) antibodies. Because of their resistance to prion-related diseases such as bovine spongiform encephalopy (also known as mad cow disease), these bovines are a safer source of human antibodies for pharmaceutical uses and a safer source of agricultural products.
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Citations
38 Claims
- 1. A bovine comprising a non-naturally occurring mutation in one or both alleles of an endogenous prion nucleic acid
- 25. A bovine cell comprising a non-naturally occurring mutation in one or both alleles of an endogenous prion nucleic acid.
- 32. A method for producing a transgenic bovine cell having reduced expression of functional prion protein, comprising introducing a first prion gene targeting vector into a bovine cell under conditions that allow homologous recombination between said first vector and a first allele of an endogenous prion nucleic acid in said cell, thereby introducing a hemizygous mutation in said cell.
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37. A method for producing a transgenic bovine having reduced expression of functional prion protein, said method comprising the steps of:
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(a) inserting a cell, a chromatin mass from a cell, or a nucleus from a cell into an oocyte, wherein said cell comprises a first mutation in an endogenous prion nucleic acid; and
(b) transferring said oocyte or an embryo formed from said oocyte into the uterus of a host bovine under conditions that allow said oocyte or said embryo to develop into a fetus. - View Dependent Claims (38)
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Specification