Functional surface coating

• US 20050100675A1
• Filed: 10/13/2004
• Published: 05/12/2005
• Est. Priority Date: 06/26/2001
• Status: Active Grant

• Current Assignee: Accelerate Diagnostics, Inc.
• Original Assignee: Accelr8 Technology Corporation
• Inventors: Lochhead, Michael J., Metzger, Steven W., Mao, Guoqiano
• Granted Patent: US 7,629,029 B2
• Time in Patent Office: Days
• US Class Current: 427/384
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 33/5306 Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 428/31504 Composite [nonstructural laminate]
• CPC Class Codes: Y10T 428/31612 As silicone, silane or siloxane
• CPC Class Codes: Y10T 428/31663 As siloxane, silicone or silane
• CPC Class Codes: Y10T 428/31678 Of metal

Abstract

Compositions and methods of preparing functional thin films or surface coatings with low non-specific binding are described. The thin films contain specified functional groups and non-specific binding repellant components. The thin films are either covalently bound to or passively adsorbed to various solid substrates. The specified functional group provides specified activity for the thin film modified solid surfaces and non-specific binding repellant components significantly reduce the non-specific binding to the thin film modified solid surfaces. Non-specific binding repellant components do not affect specified functional group'"'"'s activity in the thin films. In these methods, specified functional groups are anchored to the solid substrates through a spacer. Surface coatings are also described having both non-specific protein binding properties combined with functional groups for specific binding activity thereby providing surface coating that specifically recognize target proteins but limit binding to non-specific protein.

Claims

1. A method of preparing a functional surface on a substrate, said method comprising:

providing an effective amount of active component comprising a functional group, spacer group, and a binding group;

providing an effective amount of cross-linking component;

providing an effective amount of matrix-forming component;

affixing said active component, said cross-linking component and said matrix-forming component onto said substrate, thereby forming a functional surface.

2. The method of claim 1, wherein said affixing further comprises coating said active component, said cross-linking component and said matrix forming component onto said substrate creating a coated substrate and curing said coated substrate.

3. The method of claim 2, wherein said curing is thermal activation.

4. The method of claim 1, wherein said functional group is an electrophilically active group.

5. The method of claim 1, wherein said functional group is an amine-reactive or thiol-reactive group.

6. The method of claim 1, wherein said functional group is an amine-reactive group.

7. The method of claim 1, wherein said functional group is an active succinimide ester.

8. The method of claim 1, wherein said spacer group is a hydrophilic polymer.

9. The method of claim 1, wherein said spacer group comprises polyethylene glycol.

10. The method of claim 1, wherein said spacer group comprises linear, star-shape, multi-arm, or comb-like polyethylene glycol.

11. The method of claim 1, wherein said spacer group comprises linear polyethylene glycol.

12. The method of claim 1, wherein said spacer group comprises multi-arm polyethylene glycol.

13. The method of claim 1, wherein said binding group is a silane.

14. The method of claim 1, wherein said binding group in an alkoxysilane or chlorosilane.

15. The method of claim 1, wherein said binding group in an alkoxysilane.

16. The method of claim 1, wherein said cross-linking component is an organosilane.

17. The method of claim 1, wherein said cross-linking component is an azidosilane.

18. The method of claim 1, wherein said matrix-forming component is a polyoxyethylene-based surface-active substance.

19. The method of claim 1, wherein said matrix-forming component is surface-active substance comprising polyoxyethylene sorbitan.

20. The method of claim 1, wherein said matrix-forming component is surface-active substance comprising polyoxyethylene sorbitan tetraoleate.

21. The method of claim 1, wherein said active component comprises an electrophilically active group, a hydrophilic polymer, and a silane.

22. The method of claim 1, wherein said active component comprises an amine-reactive group, a polyethylene glycol, and a silane.

23. The method of claim 1, wherein said substrate is a solid surface.

24. The method of claim 1, wherein said substrate is composed of glass, plastic, semiconductors, or metals.

25. The method of claim 1, wherein said substrate is composed of glass or plastic.

26. The method of claim 1, wherein said substrate is composed of glass.

27. A method of preparing a functional surface on a substrate, said method comprising

providing an effective amount of active component;

providing an effective amount of cross-linking component;

providing an effective amount of matrix-forming component;

affixing said active component, said cross-linking component and said matrix forming component onto said substrate, thereby forming a functional surface.