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Nucleic acid amplification and detection of Mycobacterium species

  • US 20050100915A1
  • Filed: 09/18/2003
  • Published: 05/12/2005
  • Est. Priority Date: 12/17/1999
  • Status: Active Grant
First Claim
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1. A method of detecting Mycobacterium species present in a biological sample, comprising the steps of:

  • providing a biological sample containing nucleic acid from at least one Mycobacterium species comprising a Mycobacterium 16S ribosomal RNA (rRNA) or DNA encoding Mycobacterium 16S rRNA;

    amplifying the Mycobacterium 16S rRNA or Mycobacterium DNA in an in vitro nucleic acid amplification mixture comprising at least one polymerase activity, and a combination of at least two primers having sequences selected from the group consisting of a first primer of SEQ ID NO;

    11 and a second primer that is an oligonucleotide consisting of 19 to 25 bases containing 18 contiguous bases of SEQ ID NO;

    24 and three to seven bases 5′

    to the 18 contiguous bases of SEQ ID NO;

    24 to produce amplified Mycobacterium nucleic acid; and

    detecting the amplified Mycobacterium nucleic acid by detecting a label associated with the amplified Mycobacterium nucleic acid.

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