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Short cycle methods for sequencing polynucleotides

  • US 20050100932A1
  • Filed: 05/24/2004
  • Published: 05/12/2005
  • Est. Priority Date: 11/12/2003
  • Status: Active Grant
First Claim
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1. A method for sequencing a nucleic acid template, the method comprising the steps of:

  • (a) exposing a nucleic acid template to a primer capable of hybridizing to said template and a polymerase capable of catalyzing nucleotide addition to said primer;

    (b) adding a labeled nucleotide for a predetermined time, said predetermined time being coordinated with an amount of polymerization inhibition such that on average only 0, 1, or 2 labeled nucleotides are added to said primer;

    (c) removing excess labeled nucleotide;

    (d) neutralizing label in any incorporated nucleotide;

    (e) repeating steps a, b, c, and d at least once; and

    (f) determining a sequence of said template based upon the order of incorporation of said labeled nucleotides.

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