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PUFA polyketide synthase systems and uses thereof

  • US 20050100995A1
  • Filed: 10/13/2004
  • Published: 05/12/2005
  • Est. Priority Date: 01/14/1999
  • Status: Active Grant
First Claim
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1. An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of:

  • a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of;

    SEQ ID NO;

    2, SEQ ID NO;

    3, SEQ ID NO;

    4, SEQ ID NO;

    5, SEQ ID NO;

    6, SEQ ID NO;

    8, SEQ ID NO;

    9, SEQ ID NO;

    10, SEQ ID NO;

    1, and SEQ ID NO;

    12;

    a) a nucleic acid sequence encoding a fragment of any of the amino acid sequences of (a) having at least one biological activity selected from the group consisting of enoyl-ACP reductase (ER) activity;

    acyl carrier protein (ACP) activity;

    β

    -ketoacyl-ACP synthase (KS) activity;

    acyltransferase (AT) activity;

    β

    -ketoacyl-ACP reductase (KR) activity;

    FabA-like β

    -hydroxyacyl-ACP dehydrase (DH) activity;

    non-FabA-like dehydrase activity;

    chain length factor (CLF) activity;

    malonyl-CoA;

    ACP acyltransferase (MAT) activity; and

    4′

    -phosphopantetheinyl transferase (PPTase) activity;

    b) a nucleic acid sequence encoding an amino acid sequence that is at least about 65% identical to SEQ ID NO;

    2 or SEQ ID NO;

    8 and has at least one biological activity selected from the group consisting of;

    KS activity, MAT activity, KR activity, ACP activity, and non-FabA-like dehydrase activity;

    c) a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to SEQ ID NO;

    3 or SEQ ID NO;

    9 and has AT biological activity;

    d) a nucleic acid sequence encoding an amino acid sequence that is at least about 70% identical to SEQ ID NO;

    4 or SEQ ID NO;

    10 and has at least one biological activity selected from the group consisting of KS activity, CLF activity and DH activity;

    e) a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to SEQ ID NO;

    6 or SEQ ID NO;

    12 and has PPTase biological activity;

    f) a nucleic acid sequence encoding an amino acid sequence that is at least about 85% identical to SEQ ID NO;

    1 or at least about 95% identical to SEQ ID NO;

    5 and has ER biological activity.

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