Methods for producing ethanol from carbon substrates
First Claim
Patent Images
1. A method for producing an alcohol as an end-product comprising the steps of:
- a) contacting a biomass carbon substrate which includes raw starch and at least two substrate-converting glucoamylase enzymes to produce an intermediate comprising pentoses. hexoses or combinations thereof;
wherein one substrate-converting glucoamylase is a raw starch hydrolyzing (RSH) glucoamylase; and
b) contacting said intermediate with at least one intermediate-converting enzyme produced by an ethanologenic microorganism, wherein said intermediate is substantially all converted by said intermediate-converting enzyme to said alcohol end-product and the presence of the alcohol end-product does not inhibit the further production of said alcohol end-product.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction of the substrate. In particularly preferred embodiments, the present invention provides means for the production of ethanol.
-
Citations
80 Claims
-
1. A method for producing an alcohol as an end-product comprising the steps of:
-
a) contacting a biomass carbon substrate which includes raw starch and at least two substrate-converting glucoamylase enzymes to produce an intermediate comprising pentoses. hexoses or combinations thereof;
wherein one substrate-converting glucoamylase is a raw starch hydrolyzing (RSH) glucoamylase; and
b) contacting said intermediate with at least one intermediate-converting enzyme produced by an ethanologenic microorganism, wherein said intermediate is substantially all converted by said intermediate-converting enzyme to said alcohol end-product and the presence of the alcohol end-product does not inhibit the further production of said alcohol end-product. - View Dependent Claims (38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57)
-
-
2-37. -37. (canceled)
-
58. A method for producing ethanol as an end-product comprising the steps of:
-
a) contacting a biomass carbon substrate, which includes raw starch, with at least two substrate-converting enzymes to produce an intermediate which includes hexoses, pentoses and combinations thereof, wherein the first substrate-converting enzyme is a starch hydrolyzing glucoamylase (RSH) enzyme and the second substrate-converting enzyme is an alpha amylase;
b) contacting said intermediate with at least one intermediate-converting enzyme obtained from an ethanologenic microorganism, wherein the intermediate is substantially all converted by said intermediate-converting enzyme to an ethanol end-product; and
the presence of the ethanol end-product does not inhibit the further production of said ethanol. - View Dependent Claims (59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 80)
-
-
73. A one-step method for producing ethanol comprising,
a) adding to a conversion media a raw starch containing carbon substrate, b) contacting the carbon substrate with a raw starch hydrolyzing (RSH) glucoamylase obtained from a strain of Rhizopus, Humicola or Aspergillus and a second glucoamylase obtained from a strain of Aspergillus or Rhizopus, to produce an intermediate comprising hexoses, pentoses and combinations thereof, and c) converting the intermediate to ethanol in the presence of an ethanol producer in said conversion media, wherein the intermediate is substantially all bioconverted to ethanol and the presence of the ethanol does not inhibit the further production of said ethanol in the culture.
Specification