Compartmentalized device for cell culture, cell processing, and sample dialysis

• US 20050101009A1
• Filed: 11/10/2004
• Published: 05/12/2005
• Est. Priority Date: 11/10/2003
• Status: Active Grant

• Current Assignee: Wheaton Industries Inc. (DURAN Group Holding GmbH)
• Original Assignee: Wilson Wolf Manufacturing Corporation
• Inventors: Wilson, John R., Page, Douglas A., Robeck, Alison, Welch, Dan
• Granted Patent: US 7,799,560 B2
• Time in Patent Office: Days
• US Class Current: 435/295.300
• CPC Class Codes: C12M 23/08 Flask, bottle or test tube
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12M 27/12 Roller bottles; Roller tubes

Abstract

A versatile compartmentalized cell culture device, with a selectively permeable membrane separating the compartments, provides many attributes relative to traditional devices. It can be configured for high-density cell culture, co-culture, and sample dialysis while rolling or standing still. It can also be configured for continuous movement of liquid between compartments. The wide combination of attributes not found in other membrane based cell culture and bioprocessing devices includes more cell capacity, more cell secreted product capacity, higher cell and product density, increased medium capacity, minimized use of exogenous growth factors, compatibility with standard cell culture equipment and protocols, increased scale up efficiency, capacity to function when rolling or standing still, capacity for perfusion without the need for pumps, and more efficient sample dialysis.

Claims

1. A compartmentalized device including:

(a) a device housing

(b) a basal medium compartment comprised of a semi-permeable membrane

(c) a cell culture compartment

(d) a basal medium compartment access port

(e) a cell culture compartment access port

(f) said basal medium compartment being less than the length of said cell culture compartment.