Method and apparatus for determining absorption of electromagnetic radiation by a material

• US 20050105095A1
• Filed: 10/07/2002
• Published: 05/19/2005
• Est. Priority Date: 10/09/2001
• Status: Abandoned Application

• Current Assignee: IntelliDx, Inc.
• Original Assignee: IntelliDx, Inc.
• Inventors: Pesach, Benny, Bitton, Gabriel, Nagar, Ron, Ashkenazi, Shai
• Application Number:
  US10/491,505
• Publication Number:
  US 20050105095A1
• Time in Patent Office: Days
• US Class Current: 356/432
• CPC Class Codes: G01N 2021/6491 Measuring fluorescence and transmission; Correcting inner filter effect
• CPC Class Codes: G01N 21/1702 with opto-acoustic detection, e.g. for gases or analysing solids
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence

Abstract

A method of determining a portion of light at a given wavelength which is incident on a material that is absorbed by the material, the method comprising: transmitting a pulse of light at the given wavelength so that the pulse traverses a path through the material; generating a first signal responsive to light in the light pulse that traverses the path length without being absorbed by the material; generating a second signal responsive to energy that the material emits responsive to a portion of the light from the light pulse that is absorbed by the material as the light pulse traverses the path; and using the first and second signals to determine the absorbed portion.

Claims

1. A method of determining a portion of light at a given wavelength which is incident on a material that is absorbed by the material, the method comprising:

transmitting a pulse of light at the given wavelength so that the pulse traverses a path through the material;

generating a first signal responsive to light in the light pulse that is not absorbed but is scattered by the material relative to a direction of propagation of the at least one light pulse;

generating a second signal responsive to energy that the material emits responsive to a portion of the light from the light pulse that is absorbed by the material as the light pulse traverses the path; and

using the first and second signals to determine the absorbed portion.

2. A method according to claim 1 and comprising determining a path length for the path that the light pulse traverses and using the determined path length and the absorbed portion to determine an absorption coefficient of the material for the given wavelength.

3. A method according to claim 2 wherein the energy that the material emits comprises a pulse of acoustic energy generated in the material by a photoacoustic effect and generating the second signal comprises sensing the acoustic energy and generating a signal responsive thereto.

4. A method according to claim 3 wherein the path through the material is bounded by two surfaces and a portion of the energy in the photoacoustic pulse emitted by the material repeatedly bounces back and forth between the two surfaces and determining a path length for the path comprises determining a time period it takes for energy in the acoustic pulse to make a round trip between the surfaces and using the time period to determine the path length.

5. A method according to claim 1 wherein the energy that the material emits comprises a pulse of acoustic energy generated in the material by a photoacoustic effect and generating the second signal comprises sensing the acoustic energy and generating a signal responsive thereto.

6. A method according to claim 1 wherein the energy that the material emits comprises thermal energy and generating the second signal comprises sensing the thermal energy and generating a signal responsive thereto.

7. A method according to claim 1 wherein the energy that the material emits comprises optical energy luminesced by the material and generating the second signal comprises sensing the luminesced light and generating a signal responsive thereto.

8. A method according to claim 1 wherein generating a first signal comprises sensing optical energy in the non-absorbed light, transducing the sensed energy to acoustic energy and generating a signal responsive to the acoustic energy.

9. A method according to claim 1 wherein generating a first signal comprises sensing optical energy in the non-absorbed light, transducing optical energy in the non-absorbed light to thermal energy and generating a signal responsive to the thermal energy.

10. A method according to claim 1 comprising sensing energy originating in the light pulse as a function of time following transmission of the light pulse through the material and generating the first signal comprises generating the first signal responsive to energy sensed within a time period after transmission of the light pulse that is less than or equal to about twice a transit time of light from the light pulse over the path.

11. A method according to claim 10 wherein generating the second signal comprises generating a second signal responsive to energy sensed at time following the light pulse transmission time that is substantially later than the transit time.

12. A method according to claim 1 wherein using the first and second signals to determine the absorbed portion comprises:

using the first signal to provide an indication of energy in the light pulse that is not absorbed by the material;

using the second signal to provide an indication of energy in the light pulse that is absorbed by the material; and

using the indicated energies to determine the absorbed portion.

13. A method according to claim 12 wherein using the indicated energies to determine the absorbed portion comprises determining a quotient between the indicated energies.

14. A method according to claim 1 wherein generating the first and second signals comprises using a same detector to sense the non-absorbed light and the energy that the material emits.

15. Apparatus for determining an absorption coefficient of a material for light of a given wavelength comprising:

a light source that transmits a pulse of light at the given wavelength that traverses a path through the material;

a detector that receives light from the light pulse that is not absorbed but is scattered by the material relative to a direction of propagation of the at least one light pulse and generates a first signal responsive thereto;

a detector that receives energy emitted by the material responsive to light from the light pulse that is absorbed by the material and generates a second signal responsive to the received energy; and

a processor that receives the first and second signals and uses the signals to determine the absorption coefficient.

16. Apparatus according to claim 15 wherein the detector that receives light from the light pulse comprises an acoustic sensor that converts optical energy from the light pulse incident on the detector to acoustic energy responsive to which acoustic energy the detector generates the first signal.

17. Apparatus according to claim 15 wherein the detector that receives light from the light pulse is a thermal sensor that converts optical energy from the light pulse incident on the detector to thermal energy, responsive to which thermal energy the detector generates the first signal.

18. Apparatus according to claim 15 wherein the detector that receives energy emitted by the material comprises an acoustic sensor and the energy emitted by the material responsive to which the detector generates the second signal is acoustic energy.

19. Apparatus according to claim 15 wherein the detector that receives energy emitted by the material comprises a thermal sensor and the energy emitted by the material responsive to which the detector generates the second signal is thermal energy.

20. Apparatus according to claim 15 wherein the detector that receives light from the light pulse is the same detector that receives energy emitted by the material.

21. Apparatus according to claim 20 wherein the detector comprises an acoustic sensor and energy emitted by the material responsive to which the detector generates the second signal is a pulse of acoustic energy and wherein the acoustic sensor converts optical energy from the light pulse incident on the detector to acoustic energy to generate the first signal.

22. Apparatus according to claim 20 wherein the detector comprises a thermal detector and energy emitted by the material responsive to which the detector generates the second signal is a pulse of thermal energy and wherein the thermal sensor converts optical energy from the light pulse incident on the detector to thermal energy to generate the first signal.

23. Apparatus according to claim 18 wherein the path through the material is bounded by two surfaces and a portion of the energy in the photoacoustic pulse emitted by the material repeatedly bounces back and forth between the two surfaces and wherein the processor determines a time period required for energy in the acoustic pulse to make a round trip between the surfaces and uses the time period to determine a path length for the path and the path length to determine the absorption coefficient.

24. Apparatus according to claim 15 wherein the detector that receives energy emitted by the material is positioned so that the path that the light pulse traverses does not intersect the detector.

25. Apparatus according to claim 21 wherein the path through the material is bounded by two surfaces and a portion of the energy in the photoacoustic pulse emitted by the material repeatedly bounces back and forth between the two surfaces and wherein the processor determines a time period required for energy in the acoustic pulse to make a round trip between the surfaces and uses the time period to determine a path length for the path and the path length to determine the absorption coefficient.