Three-dimensional in-vitro spinal models and methods of analyzing substance distribution therein
First Claim
1. A three-dimensional in-vitro model apparatus of a human spine comprising:
- a model body comprising a column portion, the column portion defining a curved passageway in the shape of a human spinal canal; and
a cord structure located within the passageway of the column portion, wherein the cord structure is securable within the passageway such that an annular region is formed between a wall of the passageway and the cord structure.
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Abstract
An in-vitro model apparatus of a human spine and methods for detecting and analyzing substance distribution patterns therein. In one embodiment, the model apparatus includes a column body defining a passageway that substantially mimics the size, shape, and structure of an adult human spinal canal. Also included is a cord structure that may be located and anchored within the passageway. The cord structure substantially mimics the size, shape, and structure of an adult human spinal cord. For example, the cord structure may include connecting elements that resemble nerve roots, dentate ligaments, and the septum posticum of a human spine. The passageway of the model apparatus may be filled with a first fluid that simulates cerebro-spinal fluid (CSF), and a second fluid containing a drug (or simulated drug) may be introduced into the passageway, after which the drug'"'"'s distribution within the passageway may be analyzed.
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Citations
46 Claims
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1. A three-dimensional in-vitro model apparatus of a human spine comprising:
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a model body comprising a column portion, the column portion defining a curved passageway in the shape of a human spinal canal; and
a cord structure located within the passageway of the column portion, wherein the cord structure is securable within the passageway such that an annular region is formed between a wall of the passageway and the cord structure. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A method of characterizing a distribution of a substance in a human spinal column, the method comprising:
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introducing a second fluid comprising the substance into a first fluid, the first fluid located within an annular region of a spinal model, the spinal model comprising;
a model body comprising a column portion, the column portion defining a curved passageway in the shape of a human spinal canal; and
a cord structure located within the passageway of the column portion, wherein the cord structure is securable within the passageway such that the annular region is formed between a wall of the passageway and the cord structure; and
detecting a concentration of the substance within the first fluid at one or more locations along the passageway. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29)
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30. A three-dimensional in-vitro model of a human spine comprising:
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a column portion comprising a substantially enclosed passageway formed therein;
a cord structure securable within the passageway, wherein an annular region is formed between a wall of the passageway and the cord structure; and
one or more connecting elements spanning across the annular region between the cord structure and the wall of the passageway. - View Dependent Claims (31, 32, 33, 34)
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35. A three-dimensional in-vitro model of a human spine comprising:
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a column portion comprising a substantially enclosed passageway formed therein;
a plurality of sampling ports extending through the column portion to the passageway;
a cord structure securable within the passageway, wherein an annular region is formed between a wall of the passageway and the cord structure; and
a first fluid substantially filling the annular region. - View Dependent Claims (36, 37, 38, 39, 40)
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41. A method of characterizing a distribution of a drug in a human spinal column, the method comprising:
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filling an annular region of a spinal model with a first fluid, the spinal model comprising;
a column portion comprising a substantially enclosed passageway formed therein; and
a cord structure securable within the passageway, wherein the annular region is formed between a wall of the passageway and the cord structure;
introducing a second fluid containing the drug into the first fluid at a predetermined location along the passageway; and
detecting a concentration of the drug contained in the first fluid at one or more locations along the passageway. - View Dependent Claims (42, 43, 44, 45, 46)
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Specification