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Detection of group B streptococcus

  • US 20050106578A1
  • Filed: 11/18/2003
  • Published: 05/19/2005
  • Est. Priority Date: 11/18/2003
  • Status: Active Grant
First Claim
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1. A method for detecting the presence or absence of GBS in a biological sample from an individual, said method comprising:

  • performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of pts primers to produce a pts amplification product if a GBS pts nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of pts probes, wherein the members of said pair of pts probes hybridize within no more than five nucleotides of each other, wherein a first pts probe of said pair of pts probes is labeled with a donor fluorescent moiety and said second pts probe of said pair of pts probes is labeled with a corresponding acceptor fluorescent moiety; and

    detecting the presence or absence of fluorescence resonance energy transfer (FRET) between said donor fluorescent moiety of said first pts probe and said acceptor fluorescent moiety of said second pts probe, wherein the presence of FRET is indicative of the presence of GBS in said sample, and wherein the absence of FRET is indicative of the absence of GBS in said sample.

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