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Method for determining of nucleic acid analytes

  • US 20050106587A1
  • Filed: 06/21/2004
  • Published: 05/19/2005
  • Est. Priority Date: 12/21/2001
  • Status: Abandoned Application
First Claim
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1. A method for the determination of a nucleic acid analyte by hybridizing the analyte on a suitable nucleic acid probe immobilized on a solid phase, wherein (a) the nucleic acid analyte is incubated with the nucleic acid probe under suitable hybridization conditions to form a hybridization complex, and (b) the analyte is determined on the basis of chemical/physical measurement data, which is specifically associated with a mass increase or decrease in the hybridization complex that is due to an individual enzymatic step, whereby the measurement of the data is performed within a few seconds or minutes by at least one sensor, the sensor being an integral component of the solid phase and being selected from the group including electrode structures, field effect transistors, magnetic sensors, optical sensors, pH sensors, and combinations of the same.

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