Bioarray chip reaction apparatus and its manufacture
First Claim
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1. A method of making probe chips comprising the steps of:
- forming a plurality of probe arrays on a wafer;
separating said wafer into a plurality of chips, each of said chips comprising at least one probe array thereon; and
mating at least one of said chips to a package, said package comprising a reaction chamber, said reaction chamber comprising inlets for flowing fluid therein, said at least one probe array in fluid communication with said reaction chamber.
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Abstract
A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
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Citations
112 Claims
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1. A method of making probe chips comprising the steps of:
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forming a plurality of probe arrays on a wafer;
separating said wafer into a plurality of chips, each of said chips comprising at least one probe array thereon; and
mating at least one of said chips to a package, said package comprising a reaction chamber, said reaction chamber comprising inlets for flowing fluid therein, said at least one probe array in fluid communication with said reaction chamber.
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2-92. -92. (canceled)
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93. A method of assaying a sample, said method comprising:
- (a) contacting said sample with a surface of a first substrate to produce a substrate supported sample;
(b) contacting said substrate supported sample with a ligand array comprising two or more biopolymeric ligands immobilized on a surface of a second substrate; and
(c) reading said array to assay said sample. - View Dependent Claims (94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 110, 111, 112)
- (a) contacting said sample with a surface of a first substrate to produce a substrate supported sample;
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106. A backing element for use in a array assay, said backing element comprising:
- (a) a substrate having a first surface bounded on at least one side with a fluid barrier; and
(b) a sample component binding agent on said first surface;
wherein said backing element is dimensioned to be joined with an array to produce a defined reaction volume. - View Dependent Claims (107, 108)
- (a) a substrate having a first surface bounded on at least one side with a fluid barrier; and
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109. A system for performing an array assay, said system comprising:
- (a) an array comprising at least two different ligands; and
(b) a backing element comprising;
(i) a substrate having a first surface bounded on at least one side with a fluid barrier; and
(ii) a sample component binding agent on said first surface;
wherein said backing element is dimensioned to be joined with an array to produce a defined reaction volume.
- (a) an array comprising at least two different ligands; and
Specification