Cell culture methods and devices utilizing gas permeable materials

• US 20050106717A1
• Filed: 10/08/2004
• Published: 05/19/2005
• Est. Priority Date: 10/08/2003
• Status: Active Grant

• Current Assignee: Wilson Wolf Manufacturing Corporation
• Original Assignee: Wilson Wolf Manufacturing Corporation
• Inventors: Wilson, John R., Page, Douglas A., Robeck, Alison, Welch, Dan
• Granted Patent: US 9,255,243 B2
• Time in Patent Office: Days
• US Class Current: 435/297.500
• CPC Class Codes: C12M 23/08 Flask, bottle or test tube
• CPC Class Codes: C12M 23/24 Gas permeable parts
• CPC Class Codes: C12M 25/06 Plates; Walls; Drawers; Multilayer plates
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12N 2500/02 Atmosphere, e.g. low oxygen conditions
• CPC Class Codes: C12N 5/00 Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; plant reproduction by tissue culture techniques A01H4/00
• CPC Class Codes: C12N 5/0602 Vertebrate cells

Abstract

Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.

Claims

1. A gas permeable cell culture device, comprising a top and a bottom joined by a plurality of sides, wherein the bottom comprises a gas permeable material, and at least a portion of a side is comprised of a gas permeable material, such that cells can be cultured when said apparatus is oriented in at least two positions, vertically and horizontally.