Cell culture methods and devices utilizing gas permeable materials
First Claim
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1. A gas permeable cell culture device, comprising a top and a bottom joined by a plurality of sides, wherein the bottom comprises a gas permeable material, and at least a portion of a side is comprised of a gas permeable material, such that cells can be cultured when said apparatus is oriented in at least two positions, vertically and horizontally.
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Abstract
Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.
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1 Claim
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1. A gas permeable cell culture device, comprising a top and a bottom joined by a plurality of sides, wherein the bottom comprises a gas permeable material, and at least a portion of a side is comprised of a gas permeable material, such that cells can be cultured when said apparatus is oriented in at least two positions, vertically and horizontally.
Specification