Method and system for ultrasonic tagging of fluorescence
First Claim
1. A method for localization of fluorescence in a scattering medium, the method comprising the steps of:
- illuminating the scattering medium with an excitation light to excite the fluorescence;
modulating a portion of the emitted light from the fluorescence within the scattering medium using an ultrasonic pulse;
detecting the modulated optical signal at a surface of the scattering medium.
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Abstract
A method and system for localization of fluorescence in a scattering medium such as a biological tissue are provided. In comparison to other optical imaging techniques, this disclosure provides for improved spatial resolution, decreased computational time for reconstructions, and allows anatomical and functional imaging simultaneously. The method including the steps of illuminating the scattering medium with an excitation light to excite the fluorescence; modulating a portion of the emitted light from the fluorescence within the scattering medium using an ultrasonically induced variation of material properties of the scattering medium such as the refractive index; detecting the modulated optical signal at a surface of the scattering medium; and reconstructing a spatial distribution of the fluorescence in the scattering medium from the detected signal.
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Citations
24 Claims
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1. A method for localization of fluorescence in a scattering medium, the method comprising the steps of:
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illuminating the scattering medium with an excitation light to excite the fluorescence;
modulating a portion of the emitted light from the fluorescence within the scattering medium using an ultrasonic pulse;
detecting the modulated optical signal at a surface of the scattering medium. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for localization of fluorescence in a scattering medium, the method comprising the steps of:
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illuminating the scattering medium with an excitation light to excite the fluorescence;
modulating the excitation light using an ultrasonic pulse;
detecting a modulated optical signal emitted from the fluorescence at a surface of the scattering medium.
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15. A system for localization of fluorescence in a scattering medium comprising:
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an excitation light source for illuminating the scattering medium;
a fluorescent dye within a preselected region of the scattering medium for absorbing and emitting light in the NIR (near infrared) region of the light spectrum;
an ultrasonic scanning system for generating ultrasonic pulses, wherein the ultrasonic pulses modulate the emitted light from the fluorescent dye; and
an optical detection system for detecting the modulated light. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23)
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24. An imaging system for localization of a preselected object in a scattering medium comprising:
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an excitation light source for illuminating the scattering medium;
a fluorescent dye injected into the preselected object for absorbing and emitting light in the NIR (near infrared) region of the light spectrum;
an ultrasonic scanning system for generating ultrasonic pulses, wherein the ultrasonic pulses modulate the emitted light from the fluorescent dye;
an optical detection system for detecting the modulated light; and
a data processing system acquiring the detected optical signal and generating a spatial distribution of the preselected object in the scattering medium based on the detected optical signals.
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Specification