Novel device, system and method for fluorescence detection

• US 20050109951A1
• Filed: 12/26/2002
• Published: 05/26/2005
• Est. Priority Date: 12/27/2001
• Status: Abandoned Application

• Current Assignee: Inverness Medical Switzerland GmbH (Abbott Laboratories)
• Original Assignee: Inverness Medical Switzerland GmbH (Abbott Laboratories)
• Inventors: Fish, Falk, Faktor, Ouriel, Greenberg, Zvi
• Application Number:
  US10/500,186
• Publication Number:
  US 20050109951A1
• Time in Patent Office: Days
• US Class Current: 250/461.100
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2021/757 using immobilised reagents
• CPC Class Codes: G01N 2021/7759 Dipstick; Test strip
• CPC Class Codes: G01N 2021/7763 Sample through flow
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 2201/0221 Portable; cableless; compact; hand-held
• CPC Class Codes: G01N 2201/0222 Pocket size
• CPC Class Codes: G01N 2201/0245 with insertable-removable part
• CPC Class Codes: G01N 2201/062 LED's
• CPC Class Codes: G01N 2201/0628 Organic LED [OLED]
• CPC Class Codes: G01N 33/549 with antigen or antibody entrapped within the carrier

Abstract

A device, system and method for portable fluorescence detection. The portable device of the present invention features a low power light, in which a wavelength range is defined as at least one wavelength of light. The light source is preferably highly energy efficient, such that a majority of the electrical power which is consumed is then converted into transmitted light. The emitted light from the excited fluorophore is then preferably detected with any low cost and low power photodetector. Although optionally a highly sensitive optical detector may be used, preferably fluorescence is detected with any light sensing device, such as a regular photodiode or a CCD (charge-coupled device) sensor for example.

Claims

1. A portable device for detection of fluorescence in a sample containing a fluorophore, comprising:

(a) a light source for emitting light for exciting the fluorophore, wherein said light is of a defined wavelength range; and

(b) a photodetector for detecting emitted light from the excited fluorophore.

2. The device of claim 1, wherein said light source is characterized by being at least one of a low power or a low cost.

3. The device of claim 2, wherein said light source comprises a low power light source having a power consumption not greater than about 500 mW.

4. The device of claim 3, wherein said power consumption is less than about 200 mW.

5. The device of claim 4, wherein said power consumption is less than about 120 mW.

6. The device of claim 1, wherein said light source comprises a LED (light emitting diode).

7. The device of claim 6, wherein said light source comprises a LED having a luminous intensity from about 1 mCD to about 10 CD.

8. The device of claim 7, wherein said luminous intensity is from about 10 mCD to about 1 CD.

9. The device of claim 1, wherein said light source emits a colored light.

10. The device of claim 1, wherein said light source emits at least one of UV (ultraviolet) light or infra-red light.

11. The device of claim 1, wherein said light source emits a colored light through an alteration and/or an addition to said light source.

12. The device of claim 1, wherein said light source emits a colored light, the device further comprising a filter for filtering light emitted from said light source, wherein said colored light is formed through said filtering.

13. The device of claim 12, wherein said filter comprises a wide bandwidth excitation filter.

14. The device of claim 12, wherein said filter comprises a gelatin filter.

15. The device of any of claim 1, wherein said light source emits a colored light, and said colored light comprises at least one of light having a wavelength in the visible spectrum and light having a wavelength outside the visible spectrum.

16. The device of claim 1, wherein said light source emits a colored light, and said colored light is selected from the group consisting of ultraviolet, white, blue, green, yellow-green, yellow, orange, red, and infra-red.

17. The device of any of claim 1, further comprising a filter for filtering said emitted light from said light source.

18. The device of claim 17, wherein said filter is selected according to said defined wavelength range.

19. The device of claim 17, wherein said filter is selected according to a preferred wavelength or wavelengths for exciting the fluorophore.

20. The device of claim 1, further comprising a plurality of light sources.

21. The device of claim 20, wherein said plurality of light sources is arranged in an array.

22. The device of claim 1, wherein said photodetector is of low cost and/or of low sensitivity.

23. The device of claim 1, wherein said photodetector includes one or more of any regular photodiode, a photocell, a phototransistor, a noncooled CCD (charge-coupled device), a photoresistor, a sensor photodiode, or an array thereof.

24. The device of claim 23, wherein said photodetector comprises a photodiode.

25. The device of claim 23, wherein said photodetector comprises a CCD.

26. The device of claim 1, wherein an exposure time of said photodetector is in a range of from about {fraction (1/100)} seconds to about 60 seconds.

27. The device of claim 26, wherein said exposure time is in a range of from about {fraction (1/70)} seconds to about {fraction (1/10)} seconds.

28. The device of claim 1, wherein the fluorophore emits light in a near red or infrared range.

29. The device of claim 1, further comprising a filter for filtering emitted light from the excited fluorophore.

30. The device of claim 29, wherein said filter is selected according to a wavelength or wavelengths of said emitted light from the excited fluorophore.

31. The device of claim 29, wherein the fluorophore emits light in a near red or infrared range, and said emitted light from the excited fluorophore is filtered with a 590 nm Long Pass filter.

32. A system for detection of fluorescence in a sample containing a fluorophore, comprising:

(a) a portable device according to claim 1;

and

(b) a computational device for performing a computation.

33. The system of claim 32, further comprising:

(a) a peripheral device.

34. The system of claim 33, wherein said peripheral device comprises any one or more of a display device, a printer, or a connector to a wired or wireless network, or a combination thereof.

35. The system of claim 32, further comprising a lateral flow immunochromatography device for holding the sample and optionally one or more reagents.

36. A method for detecting fluorescence in a sample containing a fluorophore, comprising:

providing a portable device according to claim 1;

entering the sample is preferably entered to said portable device;

emitting light by a light source of said portable device, thereby exciting the fluorophore; and

detecting light emitted from the excited fluorophore by a photodetector.

37. The method of claim 36, further comprising:

providing a a computational device for performing a computation.

38. The method of claim 37, further comprising:

performing one or more computations on a signal obtained from said photodetector.

39. The method of claim 38, further comprising:

displaying a result of said one or more computations.