Reduction of Contaminants In Blood and Blood Products Using Photosensitizers and Peak Wavelengths of Light

• US 20050112021A1
• Filed: 11/05/2004
• Published: 05/26/2005
• Est. Priority Date: 06/15/2000
• Status: Abandoned Application

• Current Assignee: Caridianbct Biotechnologies LLC (Baxter International Inc.)
• Original Assignee: Gambro, Inc. (DaVita Incorporated)
• Inventors: GOODRICH, Raymond P., HLAVINKA, Dennis J., GOODRICH, Laura, MCGRAW, Daniel
• Application Number:
  US10/904,361
• Publication Number:
  US 20050112021A1
• Time in Patent Office: Days
• US Class Current: 422/24
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/00 Medicinal preparations containing organic active ingredients
• CPC Class Codes: A61K 31/28 Compounds containing heavy metals
• CPC Class Codes: A61K 31/555 containing heavy metals, e.g. hemin, hematin, melarsoprol
• CPC Class Codes: A61K 31/60 Salicylic acid; Derivatives thereof
• CPC Class Codes: A61K 31/603 having further aromatic rings, e.g. diflunisal
• CPC Class Codes: A61K 31/695 Silicon compounds
• CPC Class Codes: A61K 31/7048 having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin , digitoxin or digoxin
• CPC Class Codes: A61K 31/7135 Compounds containing heavy metals
• CPC Class Codes: A61K 33/00 Medicinal preparations containing inorganic active ingredients
• CPC Class Codes: A61K 33/14 Alkali metal chlorides; Alkaline earth metal chlorides
• CPC Class Codes: A61K 33/32 Manganese; Compounds thereof
• CPC Class Codes: A61K 35/18 Erythrocytes haemoglobin A61K38/42
• CPC Class Codes: A61K 41/0057 Photodynamic therapy with a photosensitizer, i.e. agent able to produce reactive oxygen species upon exposure to light or radiation, e.g. UV or visible light; photocleavage of nucleic acids with an agent
• CPC Class Codes: A61K 41/0071 PDT with porphyrins having exactly 20 ring atoms, i.e. based on the non-expanded tetrapyrrolic ring system, e.g. bacteriochlorin, chlorin-e6, or phthalocyanines
• CPC Class Codes: A61K 41/17 by ultraviolet [UV] or infrared [IR] light, X-rays or gamma rays
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 47/52 the modifying agent being an inorganic compound, e.g. an inorganic ion that is complexed with the active ingredient
• CPC Class Codes: A61L 2/0011 using physical methods
• CPC Class Codes: A61L 2/0047 Ultraviolet radiation
• CPC Class Codes: A61L 2/0076 : using a photocatalyst or photosensitiser
• CPC Class Codes: A61L 2/0088 : Liquid substances
• CPC Class Codes: A61L 2/08 : Radiation
• CPC Class Codes: A61L 2/10 : Ultraviolet radiation
• CPC Class Codes: A61L 2/24 : Apparatus using programmed or automatic operation
• CPC Class Codes: A61L 2/26 : Accessories or devices or components used for biocidal treatment
• CPC Class Codes: A61L 2202/14 : Means for controlling sterilisation processes, data processing, presentation and storage means, e.g. sensors, controllers, programs
• CPC Class Codes: A61L 2202/22 : Blood or products thereof
• CPC Class Codes: A61M 1/3681 : by irradiation
• CPC Class Codes: A61M 1/3683 : using photoactive agents
• CPC Class Codes: C07F 11/005 : compounds without a metal-carbon linkage
• CPC Class Codes: C07F 13/005 : Compounds without a metal-carbon linkage
• CPC Class Codes: C07F 15/02 : Iron compounds
• CPC Class Codes: C07F 17/00 : Metallocenes
• CPC Class Codes: C12N 13/00 : Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

Methods and apparatuses are provided for inactivation of pathogens in fluids containing blood products. Preferred methods include the steps of adding an effective, non-toxic amount of a photosensitizer such as riboflavin to the blood product and exposing the fluid to light having a peak wavelength.

Claims

1. A method for inactivating pathogens in a fluid containing plasma comprising;

adding a photosensitizer to the fluid to form a mixture, and

exposing the mixture of the fluid and the photosensitizer to light within an approximate range of between 305-313 nm.

2. A method of claim 1 wherein the step of exposing further comprises exposing the mixture to light having a peak wavelength at 308 nm.

3. The method of claim 1 wherein the exposing step further comprises exposing the mixture to pulsed light within the approximate range.

4. The method of claim 1 wherein the photosensitizer is an endogenous photosensitizer.

5. The method of claim 1 wherein the photosensitizer is an isoalloxazine.

6. The method of claim 1 wherein the photosensitizer is riboflavin.

7. The method of claim 1 further comprising:

filtering out all light except light in the range of between 305-313 nm.

8. The method of claim 7 wherein the step of filtering comprises filtering out all light in the UV spectrum except light having a peak wavelength of approximately 308 nm.

9. The method of claim 7 wherein the step of filtering comprises filtering out all light in the UV spectrum except light having a peak wavelength of approximately 313 nm.

10. A treatment chamber for inactivating pathogens in a fluid containing plasma and a photosensitizer comprising:

at least one radiation emitting source emitting radiation at a peak wavelength of approximately 308 nm;

a support platform for holding the fluid containing plasma and photosensitizer to be irradiated; and

a control unit for controlling the radiation emitting source.

11. The treatment chamber of claim 10 wherein the radiation emitting source is capable of being pulsed.

12. The treatment chamber of claim 10 wherein the control unit further controls the movement of the support platform.

13. The treatment chamber of claim 12 wherein the support platform is capable of movement in multiple directions within the treatment chamber.

14. The treatment chamber of claim 10 wherein the support platform is made of photopermeable material.

15. The treatment chamber of claim 10 wherein the chamber further comprises at least one reflective surface.

16. The treatment chamber of claim 10 wherein the support platform includes a reflective surface.

17. The treatment chamber of claim 11 and claim 12 wherein the control unit further moves the support platform in coordination with the radiation pulses.

18. The treatment chamber of claim 10 wherein the radiation emitting source further comprises an array containing a plurality of discrete lights.

19. The treatment chamber of claim 18 wherein the array containing a plurality of discrete lights further comprises a plurality of LEDs.

20. A method of irradiating plasma in a fluid contained within a photopermeable bag comprising the steps of:

adding riboflavin to the bag in an amount necessary to inactivate any pathogens contained in the fluid;

exposing the bag containing at least the fluid and riboflavin to a peak wavelength of light; and

mixing the contents of the bag during the exposing step.

21. A method of irradiating a fluid containing plasma comprising the steps of:

adding an amount of photosensitizer to the fluid necessary to inactivate any pathogens contained in the fluid;

exposing the fluid and photosensitizer to light at a wavelength of approximately 308 nm; and

mixing the fluid and photosensitizer during the exposing step to expose the majority of the fluid to the light.

22. A method of irradiating a blood product comprising the steps of:

a) adding an amount of photosensitizer necessary to inactivate any pathogens contained in the blood product;

b) pulsing a radiation source having a peak wavelength of light of approximately 308 nm to expose the blood product and photosensitizer to radiation at the peak wavelength;

c) pulsing the radiation source off to stop exposure of the blood product and photosensitizer to radiation;

d) mixing the blood product and photosensitizer during the step of pulsing the radiation source off; and

e) repeating steps b), c) and d).