Anthrax antigenic compositions

• US 20050112145A1
• Filed: 11/01/2002
• Published: 05/26/2005
• Est. Priority Date: 11/01/2001
• Status: Abandoned Application

• Current Assignee: Health Protection Agency (Government of United Kingdom)
• Original Assignee: Health Protection Agency (Government of United Kingdom)
• Inventors: Hudson, Michael John, Robinson, Andrew, Hallis, Bassam, Silman, Nigel, Penn, Charles
• Application Number:
  US10/494,384
• Publication Number:
  US 20050112145A1
• Time in Patent Office: Days
• US Class Current: 424/235.100
• CPC Class Codes: A61K 39/07 Bacillus
• CPC Class Codes: A61P 31/04 Antibacterial agents

Abstract

An antigenic pharmaceutical composition is provided comprising Protective Antigen (PA) and Lethal Factor (LF), wherein said PA and/or LF lacks a functional binding site, thereby preventing said PA and LF from binding together via said binding site or thereby preventing said PA from binding to a native PA cell receptor via said binding site, and wherein said composition is substantially non-toxic to animal cells. The composition is for preventing or minimising anthrax toxicity in mammals, preferably in humans. Also provided are DNA and RNA based vaccines encoding the antigenic components of said pharmaceutical composition. The present specification also describes antibodies that bind to at least one of PA, LF or EF, which binding thereby prevents:—(i) PA from binding to LF or EF, or to a native PA cell receptor; or (ii) LF from binding to PA; or (iii) EF from binding to PA.

Claims

1-21. -21. (canceled)

22. An antigenic pharmaceutical composition comprising 83 kDa Protective Antigen (PA) and Lethal Factor (LF), wherein PA lacks a functional binding site for a native PA cell receptor thereby preventing said PA from binding to said native cell receptor, and/or PA lacks a functional furin cleavage site thereby preventing said PA from in vivo cleavage by furin to form 63 kDa PA, and wherein said composition is substantially non-toxic to animal cells.

23. A composition according to claim 22, wherein the PA lacks said functional binding site for a native PA cell receptor, and wherein the PA lacks said functional furin cleavage site.

24. A composition according to claim 22, wherein said composition is substantially free of Lethal Toxin (LT) and Oedema Toxin (ET) activity.

25. A composition according to claim 22, wherein each of PA and LF lacks a functional binding site.

26. A composition according to claim 22, wherein PA lacks a functional binding site for LF thereby preventing said PA and LF from binding together via said binding site.

27. A composition according to claim 22, wherein LF lacks a functional binding site for PA thereby preventing said LF and PA from binding together via said binding site.

28. A composition according to claim 22, wherein said composition further comprises Oedema Factor (EF).

29. A composition according to claim 28, wherein said EF lacks a functional binding site for PA thereby preventing said EF and PA from binding together via said binding site.

30. A composition according to claim 22, wherein said LF has at least 30% metalloprotease activity when compared weight by weight with native LF.

31. A composition according to claim 28, wherein said EF has at least 30% adenylyl cyclase activity when compared weight by weight with native EF.

32. A composition according to claim 22, wherein said composition further comprises Sap 1 and/or EA1.

33. A composition according to claim 22, wherein PA and LF are each present at a concentration of 1-60 μ

g/ml.

34. The composition according to claim 33, wherein PA and LF are each present at a concentration of 2-20 μ

g/ml.

35. A composition according to claim 22, wherein the composition comprises PA and LF in weight ratios of 1:

3 to 3;

1.

36. The composition according to claim 35, wherein the composition comprises PA and LF in weight ratios of 1:

1.5 to 1.5;

1.

37. A recombinant method for preparing an antigenic composition according to claim 22, said method comprising:

expressing in a B. anthracis host cell a nucleic acid construct encoding said PA, and recovering the expressed PA;

expressing in a B. anthracis host cell a nucleic acid construct encoding said LF, and recovering the expressed LF; and

combining the PA and LF.

38. The method of claim 37, further comprising expressing in a B. anthracis host cell a nucleic acid construct encoding EF, and recovering the expressed EF;

and

combining the EF with the PA and LF.

39. One or more DNA plasmid(s) that encode PA and LF as defined in claim 22;

and wherein said plasmid(s) comprise a eukaryotic promoter that is operably linked to and, in use, drives expression of said PA and LF.

40. The DNA plasmid(s) of claim 39, further encoding EF.

41. One or more RNA vector(s) that encode PA and LF as defined in claim 22;

and wherein the vector(s) comprise an integration site for a chromosome of a mammal host cell.

42. The RNA vector(s) of claim 41, further encoding EF.

43. A method of preventing or minimizing anthrax toxicity in a mammal, comprising administering to said mammal a composition according to claim 22, DNA plasmid(s) according to claim 39, or RNA vector(s) according to claim 41.

44. The method of claim 43, wherein said mammal is a human.

45. A method of preventing or minimizing anthrax toxicity in a mammal, comprising administering to said mammal a PA as defined in claim 22, wherein said PA is administered prior to, simultaneously with or subsequent to LF as defined in claim 22.

46. The method of claim 45, wherein said mammal is a human.