Microarray comprising a substrate having two-dimensional grating and method of detecting target molecule by using the same

• US 20050112651A1
• Filed: 10/19/2004
• Published: 05/26/2005
• Est. Priority Date: 11/20/2003
• Status: Abandoned Application

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Lee, Younghwan, Lee, Injae, Kim, Younghun, Sim, Huijun, Kwon, Youngnam, Ma, Jangseok, Wang, Fu, Lee, Soosuk
• Application Number:
  US10/968,540
• Publication Number:
  US 20050112651A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2523/313 Irradiation, e.g. UV irradiation
• CPC Class Codes: C12Q 2565/518 characterised by the immobilisation of the nucleic acid sample or target
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A microarray including a substrate having a first diffraction grating and a second diffraction grating formed perpendicularly to each other is provided. Also, a method of detecting a target molecule in a sample is provided. The method includes: placing a sample containing a labelled target molecule on a diffraction grating on the above microarray to react with a probe molecule immobilized on the diffraction grating; irradiating a first electromagnetic wave to a product of a reaction between the target molecule and the probe molecule; and detecting a second electromagnetic wave emitted from the labelled probe molecule.

Claims

1. A microarray comprising a substrate having a first diffraction grating and a second diffraction grating formed perpendicularly to each other.

2. The microarray of claim 1, wherein the grating has a square, trapezoidal, triangular, sine wave, or blaze shape.

3. The microarray of claim 1, wherein a surface of the grating is coated with a high refractive index material having a higher refractive index than the substrate.

4. The microarray of claim 3, wherein the high refractive index material is selected from the group consisting of TiO₂, Ta₃O₅, HfO₂, ZrO₂, ZnO, and Nb₂O₅.

5. The microarray of claim 1, wherein period of the grating is 300-600 nm.

6. The microarray of claim 1, wherein the microarray is a polynucleotide microarry or a protein micro array.

7. A method of detecting a target molecule in a sample, the method comprising:

placing a sample containing a labelled target molecule on a diffraction grating on a microarray of claim 1 to react with a probe molecule immobilized on the diffraction grating;

irradiating a first electromagnetic wave onto a product of a reaction between the target molecule and the probe molecule; and

detecting a second electromagnetic wave emitted from the labelled probe molecule.

8. The method of claim 7, wherein the microarray is a polynucleotide microarray or a protein microarray.

9. The method of claim 7, wherein the label is a fluorescent label.

10. The method of claim 7, wherein the grating has a square, trapezoidal, triangular, sine wave, or blaze shape.

11. The method of claim 7, wherein a surface of the grating is coated with a high refractive index material having a higher refractive index than the substrate.

12. The method of claim 11, wherein the high refractive index material is selected from the group consisting of TiO₂, Ta₃O₅, HfO₂, ZrO₂, ZnO, and Nb₂O₅.

13. The method of claim 7, wherein period of the grating is 300-600 nm.

14. The method of claim 7, wherein the microarray is a polynucleotide microarry or a protein microarray.