System, method, and product for dynamic noise reduction in scanning of biological material
First Claim
1. A method for analyzing molecules, comprising the steps of:
- (1) directing an excitation beam to a plurality of pixel locations on a surface having a plurality of probe locations, each probe location including one or more probe molecules;
(2) determining one or more representative excitation values, each related to a value of the excitation beam as directed to at least one of the plurality of pixel locations;
(3) detecting an emission signal having one or more emission values;
(4) correlating each of the one or more emission values with one or more of the representative excitation values;
(5) providing at least one excitation reference value;
(6) comparing the excitation reference value to at least one representative excitation value, thereby generating a normalization factor;
(7) adjusting at least one emission value based, at least in part, on the normalization factor; and
(8) analyzing at least one probe location based, at least in part, on the at least one adjusted emission value.
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Accused Products
Abstract
Systems and methods are described for processing an emission signal, such as a fluorescent signal, to compensate for noise in an excitation beam, such as a laser beam. As one example, a scanning system is described that includes an excitation signal generator that provides an excitation signal having one or more representative excitation values representative of an excitation beam; an excitation reference provider that provides at least one excitation reference value; a normalization factor generator that compares the excitation reference value to at least one representative excitation value, thereby generating a normalization factor; and a comparison processor that adjusts at least one emission value corresponding to the at least one representative excitation value based, at least in part, on the normalization factor.
45 Citations
21 Claims
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1. A method for analyzing molecules, comprising the steps of:
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(1) directing an excitation beam to a plurality of pixel locations on a surface having a plurality of probe locations, each probe location including one or more probe molecules;
(2) determining one or more representative excitation values, each related to a value of the excitation beam as directed to at least one of the plurality of pixel locations;
(3) detecting an emission signal having one or more emission values;
(4) correlating each of the one or more emission values with one or more of the representative excitation values;
(5) providing at least one excitation reference value;
(6) comparing the excitation reference value to at least one representative excitation value, thereby generating a normalization factor;
(7) adjusting at least one emission value based, at least in part, on the normalization factor; and
(8) analyzing at least one probe location based, at least in part, on the at least one adjusted emission value.
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2. A method comprising the steps of:
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(1) directing an excitation beam to a plurality of pixel locations on a substrate;
(2) determining one or more representative excitation values, each related to a value of the excitation beam as directed to at least one of the plurality of pixel locations;
(3) detecting an emission signal having one or more emission values;
(4) correlating each of the one or more emission values with one or more of the representative excitation values;
(5) providing at least one excitation reference value;
(6) comparing the excitation reference value to at least one representative excitation value, thereby generating a normalization factor; and
(7) adjusting at least one emission value based, at least in part, on the normalization factor. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21-38. -38. (canceled)
Specification