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Membrane-based lateral flow assay devices that utilize phosphorescent detection

  • US 20050112703A1
  • Filed: 11/21/2003
  • Published: 05/26/2005
  • Est. Priority Date: 11/21/2003
  • Status: Abandoned Application
First Claim
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1. A method for detecting the presence or quantity of an analyte residing in a test sample, said method comprising:

  • i) providing a lateral flow assay device that comprises a porous membrane in fluid communication with detection probes, said detection probes comprising a phosphorescent label encapsulated within a matrix, wherein said porous membrane defines a detection zone within which is immobilized a capture reagent that is configured to bind to said detection probes or complexes thereof;

    ii) contacting said detection probes with the test sample;

    iii) allowing said detection probes and the test sample to flow to said detection zone;

    iv) exciting said phosphorescent label at said detection zone to generate an emitted detection signal; and

    v) measuring the intensity of the detection signal, wherein the amount of the analyte within the test sample is proportional to the intensity of the detection signal.

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