Membrane-based lateral flow assay devices that utilize phosphorescent detection
First Claim
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1. A method for detecting the presence or quantity of an analyte residing in a test sample, said method comprising:
- i) providing a lateral flow assay device that comprises a porous membrane in fluid communication with detection probes, said detection probes comprising a phosphorescent label encapsulated within a matrix, wherein said porous membrane defines a detection zone within which is immobilized a capture reagent that is configured to bind to said detection probes or complexes thereof;
ii) contacting said detection probes with the test sample;
iii) allowing said detection probes and the test sample to flow to said detection zone;
iv) exciting said phosphorescent label at said detection zone to generate an emitted detection signal; and
v) measuring the intensity of the detection signal, wherein the amount of the analyte within the test sample is proportional to the intensity of the detection signal.
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Abstract
A lateral flow, membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes phosphorescence to detect the signals generated by excited phosphorescent labels. The labels may have a long emission lifetime so that background interference from many sources, such as scattered light and autofluorescence, is practically eliminated during detection. In addition, the phosphorescent labels may be encapsulated within particles to shield the labels from quenchers, such as oxygen or water, which might disrupt the phosphorescent signal.
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Citations
63 Claims
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1. A method for detecting the presence or quantity of an analyte residing in a test sample, said method comprising:
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i) providing a lateral flow assay device that comprises a porous membrane in fluid communication with detection probes, said detection probes comprising a phosphorescent label encapsulated within a matrix, wherein said porous membrane defines a detection zone within which is immobilized a capture reagent that is configured to bind to said detection probes or complexes thereof;
ii) contacting said detection probes with the test sample;
iii) allowing said detection probes and the test sample to flow to said detection zone;
iv) exciting said phosphorescent label at said detection zone to generate an emitted detection signal; and
v) measuring the intensity of the detection signal, wherein the amount of the analyte within the test sample is proportional to the intensity of the detection signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method for detecting the presence or quantity of an analyte residing in a test sample, said method comprising:
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i) providing a lateral flow assay device that comprises a porous membrane in fluid communication with detection probes, said detection probes comprising a phosphorescent metal complex encapsulated within a particle matrix, said phosphorescent metal complex comprising a ligand and a metal selected from the group consisting of ruthenium, rhenium, osmium, platinum, palladium, and combinations thereof, said phosphorescent metal complex having a phosphorescence emission lifetime of greater than about 1 microsecond, wherein said porous membrane defines a detection zone within which is immobilized a capture reagent configured to bind to complexes of said detection probes;
ii) contacting said detection probes with the test sample;
iii) allowing said detection probes and the test sample to flow to said detection zone;
iv) exciting said phosphorescent metal complex at said detection zone to generate an emitted detection signal; and
v) measuring the intensity of the detection signal, wherein the amount of the analyte within the test sample is proportional to the intensity of the detection signal. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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- 44. A lateral flow assay device for detecting the presence or quantity of an analyte residing in a test sample, said assay device comprising a porous membrane in fluid communication with detection probes, said detection probes comprising a phosphorescent metal complex encapsulated within a matrix, wherein said porous membrane defines a detection zone within which a capture reagent is immobilized that is configured to bind to said detection probes or complexes thereof to generate a detection signal, wherein the amount of the analyte in the test sample is proportional to the intensity of said detection signal.
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