Product and process for transformation of thraustochytriales microorganisms
First Claim
1. An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of:
- a. a nucleic acid sequence encoding a protein having an amino acid sequence selected from the group consisting of SEQ ID NO;
15, SEQ ID NO;
19, SEQ ID NO;
22 and SEQ ID NO;
24, wherein said protein is an acetolactate synthase;
b. a nucleic acid sequence encoding a protein having an amino acid sequence that is at least about 75% identical to an amino acid sequence of (a), wherein said protein is an acetolactate synthase; and
, c. a nucleic acid sequence that is fully complementary to said nucleic acid sequence of (a) or (b).
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Abstract
Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.
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Citations
35 Claims
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1. An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of:
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a. a nucleic acid sequence encoding a protein having an amino acid sequence selected from the group consisting of SEQ ID NO;
15, SEQ ID NO;
19, SEQ ID NO;
22 and SEQ ID NO;
24, wherein said protein is an acetolactate synthase;
b. a nucleic acid sequence encoding a protein having an amino acid sequence that is at least about 75% identical to an amino acid sequence of (a), wherein said protein is an acetolactate synthase; and
,c. a nucleic acid sequence that is fully complementary to said nucleic acid sequence of (a) or (b). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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14. A recombinant vector for transformation of microorganisms of the Order Thraustochytriales, comprising a nucleic acid sequence encoding an acetolactate synthase that confers reduced sensitivity to compounds selected from the group consisting of:
- sulfonylurea compounds, imidazolinone-class inhibitors, and pyrimidinyl oxybenzoates, onto a microorganism of the order Thraustochytriales, wherein said acetolactate synthase has an amino acid sequence selected from the group consisting of;
a. an amino acid sequence selected from the group consisting of SEQ ID NO;
19, SEQ ID NO;
22 and SEQ ID NO;
24; and
,b. an amino acid sequence that is at least about 75% identical to an amino acid sequence of (a);
wherein said nucleic acid sequence encoding an acetolactate synthase is operatively linked to a transcription control sequence. - View Dependent Claims (15, 16, 17, 18, 19, 20)
- sulfonylurea compounds, imidazolinone-class inhibitors, and pyrimidinyl oxybenzoates, onto a microorganism of the order Thraustochytriales, wherein said acetolactate synthase has an amino acid sequence selected from the group consisting of;
Specification