Apparatus and method for amplifying a polynucleotide

• US 20050112754A1
• Filed: 12/05/2002
• Published: 05/26/2005
• Est. Priority Date: 03/09/2002
• Status: Abandoned Application

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Yoon, Dae-Sung, Jung, Moon-Hyuck, Lee, Jae-Chang, Lee, You-Seop, Lim, Geun-Bae
• Application Number:
  US10/476,036
• Publication Number:
  US 20050112754A1
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1861 using radiation
• CPC Class Codes: B01L 2300/1883 using thermal insulation
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/54 using spatial temperature gradients
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2565/629 being a microfluidic device

Abstract

The present invention provides an apparatus for amplifying a polynucleotide, comprising a substrate, a microflow channel system disposed in the substrate and comprising a sample inlet port, a sample flow channel extending from the sample inlet port, and a polynucleotide polymerization reaction chamber in fluid communication with the sample flow channel, a first insulation groove formed around the reaction chamber, and a means for regulating a temperature of the reaction chamber. Accordingly, a multiple chamber device for amplifying a polynucleotide, comprising multiple polymerization reaction chambers formed in a substrate can be manufactured.

Claims

1. An apparatus for amplifying a polynucleotide, comprising:

a substrate;

a microflow channel system disposed in the substrate and comprising a sample inlet port, a sample flow channel extending from the sample inlet port, and a polynucleotide polymerization reaction chamber in fluid communication with the sample flow channel;

a first insulation groove formed around the reaction chamber; and

a means for regulating a temperature of the reaction chamber.

2. The apparatus of claim 1, wherein the depth of the sample flow channel and the polymerization reaction chamber are about 0.1 to 500 μ

m.

3. The apparatus of claim 1, wherein the width of the sample flow channel and the polymerization reaction chamber are about 0.1 to 500 μ

m.

4. The apparatus of claim 1, further comprising a cell lysis means for lysing a cell sample, the cell lysis means being in fluid communication with the reaction chamber.

5. The apparatus of claim 1, wherein the first groove has a width of about 0.3 mm to 3 mm.

6. The apparatus of claim 1, wherein the first groove has a depth of about 200 μ

m to 290 μ

m in case that a silicon substrate has a depth of 300 μ

m or a depth of about 200 μ

m to 490 μ

m in case that a silicon substrate has a depth of 500 μ

m.

7. An apparatus for amplifying a polynucleotide, comprising a substrate and a plurality of unit devices for amplifying a polynucleotide disposed on the substrate, each of the unit device comprising;

a microflow channel system disposed in the substrate and comprising a sample inlet port, a sample flow channel extending from the sample inlet port, and a polynucleotide polymerization reaction chamber in fluid communication with the sample flow channel;

a first insulation groove formed around the reaction chamber; and

a means for regulating a temperature of the reaction chamber.

8. The apparatus of claim 7, wherein the depth of the sample flow channel and the polymerization reaction chamber are about 0.1 to 500 μ

m.

9. The apparatus of claim 7, wherein the width of the sample flow channel and the polymerization reaction chamber are about 0.1 to 500 μ

m.

10. The apparatus of claim 7, wherein the each unit device is further comprising a cell lysis means for lysing a cell sample, the cell lysis means being in fluid communication with the reaction chamber

11. The apparatus of claim 7, wherein the first groove has a width of about 0.3 mm to 3 mm.

12. The apparatus of claim 7, wherein the first groove has a depth of about 200 μ

m to 290 μ

m in case that a silicon substrate has a depth of 300 μ

m or a depth of about 200 μ

m to 490 μ

m in case that a silicon substrate has a depth of 500 μ

m.

13. The apparatus of claim 7, further comprising a second insulation groove for defining a boundary of each unit device for amplifying a polynucleotide.

14. A method for amplifying a polynucleotide contained in a sample by conducting PCR, comprising:

preparing a biochip comprising a reaction chamber and insulation groove in a substrate;

delivering a sample polynucleotide and a reagent for a polymerization reaction; and

controlling the temperature of the reaction chamber for PCR.

15. A method for amplifying a polynucleotide contained in a sample by conducting PCR, comprising:

preparing a biochip comprising a substrate and a plurality of unit amplification devices, each unit amplification device comprising;

a microflow channel system disposed in the substrate and comprising a sample inlet port, a sample flow channel extending from the sample inlet port, and a polynucleotide polymerization reaction chamber in fluid communication with the sample flow channel;

a first insulation groove formed around the reaction chamber; and

a means for regulating a temperature of the reaction chamber,

delivering a sample polynucleotide and a reagent for a polymerization reaction to each of the reaction chambers; and

independently controlling the temperature of the reaction chambers for PCR.

16. The method of claim 15, wherein the biochip further comprising a second insulation groove defining a boundary of each unit amplification device.

17. The method of claim 15, wherein independently controlling includes independently controlling the temperature of the reaction chambers at the same time schedule.

18. The method of claim 15, wherein independently controlling includes independently controlling the temperature of the reaction chambers at a different time schedule.