In vitro culture of tissue structures

• US 20050112760A1
• Filed: 07/23/2004
• Published: 05/26/2005
• Est. Priority Date: 09/30/2002
• Status: Abandoned Application

• Current Assignee: Massachusetts Eye And Ear Infirmary (Foundation of The Massachusetts Eye and EAR Infirmary Incorporated), University Of Massachusetts
• Original Assignee: Massachusetts Eye And Ear Infirmary (Foundation of The Massachusetts Eye and EAR Infirmary Incorporated)
• Inventors: Kamil, Syed H., Eavey, Roland D., Vacanti, Martin P.
• Application Number:
  US10/898,468
• Publication Number:
  US 20050112760A1
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61L 27/3817 Cartilage-forming cells, e.g. pre-chondrocytes
• CPC Class Codes: A61L 27/3852 Cartilage, e.g. meniscus
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61L 27/58 Materials at least partially resorbable by the body
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 2533/32 Polylysine, polyornithine
• CPC Class Codes: C12N 5/0655 Chondrocytes; Cartilage

Abstract

A method for growing a tissue structure includes providing a scaffold having the shape of the tissue structure and seeding the scaffold with tissue precursor cells. The seeded scaffold is then grown in vitro under conditions and for an incubation time sufficient for the tissue precursor cells to produce tissue on the scaffold and to from a tissue structure that can be implanted into a patient without causing an immune response.

Claims

1. A method for growing a tissue structure, the method comprising:

providing a scaffold having the shape of the tissue structure, the scaffold including a biodegradable and/or non-biodegradable material;

seeding the scaffold with tissue precursor cells; and

growing the seeded scaffold in vitro under conditions and for an incubation time sufficient for the tissue precursor cells to produce tissue on the scaffold and form a tissue structure that can be implanted into a patient without causing an immune response.

2. The method of claim 1, further comprising selecting the scaffold to include one or more materials selected from the group consisting of acrylic, polyethylene, polypropylene, and polystyrene.

3. The method of claim 1, further comprising selecting the scaffold to include a plastic.

4. The method of claim 1, further comprising selecting the tissue precursor cells to include chondrocytes.

5. The method of claim 1, further comprising selecting the incubation time to be at least ten weeks.

6. A tissue structure grown by the method of claim 1.