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Microscopy imaging apparatus and method for generating an image

  • US 20050117172A1
  • Filed: 01/15/2003
  • Published: 06/02/2005
  • Est. Priority Date: 01/15/2002
  • Status: Active Grant
First Claim
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1. A method of generating an optically sectioned image of a specimen comprising the steps of:

  • illuminating the specimen with a temporally modulating, spatially periodic illumination pattern;

    imaging said specimen on a conjugate image plane;

    acquiring a plurality of signals at respective positions on said image plane, each signal corresponding to the incident light intensity at that position and having an oscillatory component caused by the modulation of the illumination pattern; and

    measuring a characteristic of the oscillatory component of each of the signals, whereby the measured characteristics when combined in their relative positions generate an optically sectioned image of the specimen.

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