Microscopy imaging apparatus and method for generating an image
First Claim
1. A method of generating an optically sectioned image of a specimen comprising the steps of:
- illuminating the specimen with a temporally modulating, spatially periodic illumination pattern;
imaging said specimen on a conjugate image plane;
acquiring a plurality of signals at respective positions on said image plane, each signal corresponding to the incident light intensity at that position and having an oscillatory component caused by the modulation of the illumination pattern; and
measuring a characteristic of the oscillatory component of each of the signals, whereby the measured characteristics when combined in their relative positions generate an optically sectioned image of the specimen.
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Abstract
A method and a microscopy imaging apparatus for generating an optically sectioned image of a specimen are provided. The method comprises the steps of: illuminating the specimen with a modulating, spatially periodic illumination pattern; imaging said specimen on a conjugate image plane; acquiring a plurality of signals at respective positions on said image plane, each signal corresponding to the incident light intensity at that position and having an oscillatory component caused by the modulation of the illumination pattern; and measuring a characteristic of the oscillatory component of each of the signals, whereby the measured characteristics when combined in their relative positions generate an optically sectioned image of the specimen.
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Citations
19 Claims
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1. A method of generating an optically sectioned image of a specimen comprising the steps of:
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illuminating the specimen with a temporally modulating, spatially periodic illumination pattern;
imaging said specimen on a conjugate image plane;
acquiring a plurality of signals at respective positions on said image plane, each signal corresponding to the incident light intensity at that position and having an oscillatory component caused by the modulation of the illumination pattern; and
measuring a characteristic of the oscillatory component of each of the signals, whereby the measured characteristics when combined in their relative positions generate an optically sectioned image of the specimen. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of processing optical signals to generate an optically sectioned image comprising:
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receiving data which comprises a plurality of signals previously acquired by performing the steps of (i) illuminating a specimen with a temporally modulating, spatially periodic illumination pattern, (ii) imaging said specimen on a conjugate image plane, and (iii) acquiring a plurality of signals at respective positions on said image plane, each signal corresponding to the incident light intensity at that position and having an oscillatory component caused by the modulation of the illumination pattern, and measuring a characteristic of the oscillatory component of each of the signals, whereby the measured characteristics when combined in their relative positions generate an optically sectioned image of the specimen.
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11. A microscopy imaging apparatus comprising:
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illumination means for illuminating a specimen with a temporally modulating, spatially periodic illumination pattern;
imaging means for imaging said specimen on a conjugate image plane;
acquisition means for acquiring a plurality of signals at respective positions on said image plane, each signal corresponding to the incident light intensity at that position and having an oscillatory component caused by the modulating illumination pattern; and
processor means for measuring a characteristic of the oscillatory component of each of the signals, whereby the measured characteristics when combined in their relative positions generate an optically sectioned image of the specimen. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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Specification