Methods for identifying risk of melanoma and treatments thereof
First Claim
1. A method for identifying a subject at risk of melanoma, which comprises detecting the presence or absence of one or more polymorphic variations associated with melanoma in a nucleic acid sample from a subject, wherein the polymorphic variation is detected in a nucleotide sequence selected from the group consisting of (a) the nucleotide sequence of SEQ ID NO:
- 1, 2 or 3;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c);
whereby the presence of the polymorphic variation is indicative of the subject being at risk of melanoma.
2 Assignments
0 Petitions
Accused Products
Abstract
Provided herein are methods for identifying risk of melanoma in a subject and/or subjects at risk of melanoma, reagents and kits for carrying out the methods, methods for identifying candidate therapeutics for treating melanoma, therapeutic methods for treating melanoma in a subject and compositions comprising one or more melanoma cells and one or more NRP1, NID2 or ENDO180 directed agents. These embodiments are based upon an analysis of polymorphic variations in a NRP1, NID2 or ENDO180 nucleic acid, exemplified by nucleotide sequences of SEQ ID NO: 1, 2 or 3.
17 Citations
60 Claims
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1. A method for identifying a subject at risk of melanoma, which comprises detecting the presence or absence of one or more polymorphic variations associated with melanoma in a nucleic acid sample from a subject, wherein the polymorphic variation is detected in a nucleotide sequence selected from the group consisting of
(a) the nucleotide sequence of SEQ ID NO: - 1, 2 or 3;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c);
whereby the presence of the polymorphic variation is indicative of the subject being at risk of melanoma. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- 1, 2 or 3;
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15. A method for identifying a polymorphic variation associated with melanoma proximal to an incident polymorphic variation associated with melanoma, which comprises:
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identifying a polymorphic variation proximal to the incident polymorphic variation associated with melanoma, wherein the polymorphic variation is detected in a nucleotide sequence selected from the group consisting of;
(a) the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c) comprising the polymorphic variation;
determining the presence or absence of an association of the proximal polymorphic variant with melanoma. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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23. An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
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(a) the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c); and
(e) a nucleotide sequence complementary to the nucleotide sequences of (a), (b), (c), or (d);
wherein the nucleotide sequence comprises one or more nucleotides selected from the group consisting of a thymine at chromosome position 33767168, an adenine at chromosome position 50495413, a guanine at chromosome position 61089738, a thymine at chromosome position 50497467, an adenine at position 12008 in SEQ ID NO;
1, a guanine at position 32137 in SEQ ID NO;
1, a cytosine at position 32720 in SEQ ID NO;
1, a thymine at position 43721 in SEQ ID NO;
1, a guanine at position 44339 in SEQ ID NO;
1, a thymine at position 45640 in SEQ ID NO;
1, a thymine at position 48768 in SEQ ID NO;
1, a guanine at position 74247 in SEQ ID NO;
1, an adenine at position 75828 in SEQ ID NO;
1, a thymine at position 76381 in SEQ ID NO;
1, and a guanine at position 84909 in SEQ ID NO;
1. - View Dependent Claims (24, 25, 26)
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27. A method for identifying a candidate molecule that modulates cell proliferation, which comprises:
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(a) introducing a test molecule to a system which comprises a nucleic acid comprising a nucleotide sequence selected from the group consisting of;
(i) the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(ii) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(iii) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3; and
(iv) a fragment of a nucleotide sequence of (i), (ii), or (iii);
orintroducing a test molecule to a system which comprises a protein encoded by a nucleotide sequence of (i), (ii), (iii), or (iv); and
(b) determining the presence or absence of an interaction between the test molecule and the nucleic acid or protein, whereby the presence of an interaction between the test molecule and the nucleic acid or protein identifies the test molecule as a candidate molecule that modulates cell proliferation. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34)
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35. A method for identifying a candidate therapeutic for treating melanoma, which comprises:
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(a) introducing a test molecule to a system which comprises a nucleic acid comprising a nucleotide sequence selected from the group consisting of;
(i) the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(ii) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(iii) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3; and
(iv) a fragment of a nucleotide sequence of (i), (ii), or (iii);
orintroducing a test molecule to a system which comprises a protein encoded by a nucleotide sequence of (i), (ii), (iii), or (iv); and
(b) determining the presence or absence of an interaction between the test molecule and the nucleic acid or protein, whereby the presence of an interaction between the test molecule and the nucleic acid or protein identifies the test molecule as a candidate therapeutic for treating melanoma.
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36. A method for treating melanoma in a subject, which comprises contacting one or more cells of a subject in need thereof with a nucleic acid, wherein the nucleic acid comprises a nucleotide sequence selected from the group consisting of:
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(a) the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c); and
(e) a nucleotide sequence complementary to the nucleotide sequences of (a), (b), (c), or (d);
whereby contacting the one or more cells of the subject with the nucleic acid treats melanoma in the subject. - View Dependent Claims (37, 38)
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39. A method for treating melanoma in a subject, which comprises contacting one or more cells of a subject in need thereof with a protein, wherein the protein is encoded by a nucleotide sequence which comprises a polynucleotide sequence selected from the group consisting of:
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(a) the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c);
whereby contacting the one or more cells of the subject with the protein treats melanoma in the subject.
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40. A method for treating melanoma in a subject, which comprises:
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detecting the presence or absence of one or more polymorphic variations associated with melanoma in a nucleic acid sample from a subject, wherein the polymorphic variation is detected in a nucleotide sequence selected from the group consisting of;
(a) the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c) comprising the polymorphic variation; and
administering a melanoma treatment to a subject in need thereof based upon the presence or absence of the one or more polymorphic variations in the nucleic acid sample. - View Dependent Claims (41, 42, 43, 44, 45)
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46. A method for preventing melanoma in a subject, which comprises:
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detecting the presence or absence of one or more polymorphic variations associated with melanoma in a nucleic acid sample from a subject, wherein the polymorphic variation is detected in a nucleotide sequence selected from the group consisting of;
(a) the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3,; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c) comprising the polymorphic variation; and
administering a melanoma preventative to a subject in need thereof based upon the presence or absence of the one or more polymorphic variations in the nucleic acid sample. - View Dependent Claims (47, 48, 49, 50)
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51. A method of targeting information for preventing or treating melanoma to a subject in need thereof, which comprises:
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detecting the presence or absence of one or more polymorphic variations associated with melanoma in a nucleic acid sample from a subject, wherein the polymorphic variation is detected in a nucleotide sequence selected from the group consisting of;
(a) the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1, 2 or 3;
(c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO;
1,2 or 3; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c) comprising the polymorphic variation comprising the polymorphic variation; and
directing information for preventing or treating melanoma to a subject in need thereof based upon the presence or absence of the one or more polymorphic variations in the nucleic acid sample. - View Dependent Claims (52, 53, 54, 55, 56)
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57. A composition comprising a melanoma cell and an antibody that specifically binds to a protein, polypeptide or peptide encoded by a nucleotide sequence 90% or more identical to the nucleotide sequence of SEQ ID NO:
- 1, 2 or 3.
- View Dependent Claims (58)
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59. A composition comprising a melanoma cell and a RNA, DNA, PNA or ribozyme molecule comprising a nucleotide sequence identical to or 90% or more identical to a portion of a nucleotide sequence of SEQ ID NO:
- 1, 2 or 3.
- View Dependent Claims (60)
Specification