Compositions and methods related to mammalian Maf-A
First Claim
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1. A method of generating a β
- -like cell comprising;
a) providing an expression cassette comprising a nucleic acid sequence encoding MafA under the control of a heterologous promoter; and
b) transferring the expression cassette into a non-insulin producing cell, wherein the expression of the MafA in the cell converts the cell into a β
-like cell.
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Abstract
Various embodiments of the invention include methods of generating a β-like cell comprising providing an expression cassette comprising a nucleic acid sequence encoding MafA under the control of a heterologous promoter and transferring the expression cassette into a non-insulin producing cell, wherein the expression of the MafA in the cell converts the cell into a β-like cell.
16 Citations
41 Claims
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1. A method of generating a β
- -like cell comprising;
a) providing an expression cassette comprising a nucleic acid sequence encoding MafA under the control of a heterologous promoter; and
b) transferring the expression cassette into a non-insulin producing cell, wherein the expression of the MafA in the cell converts the cell into a β
-like cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
- -like cell comprising;
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18. A method of generating a β
- -like cell comprising;
a) providing a MafA protein comprising a nuclear localization signal; and
b) contacting a cell with a sufficient amount of the MafA protein;
wherein contacting the cell with a sufficient amount of the MafA protein converts the cell into a β
-like cell. - View Dependent Claims (19, 20, 21, 22, 23, 24)
- -like cell comprising;
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25. An implantable device for treating diabetes in a subject comprising:
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a) a receptacle suitable for holding live cells; and
b) a cell-impermeable membrane operably fixed to the receptacle so as to confine the cells within the receptacle, wherein the membrane is permeable to insulin, regulatory signals that regulate the production of insulin and other factors necessary for the survival of the cells. - View Dependent Claims (26, 27, 28)
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29. A method of providing regulated insulin production to a subject comprising providing an effective amount of a composition comprising a β
- -like cell to a subject, wherein the β
-like cell comprises an expression cassette comprising a nucleic acid sequence encoding MafA under the control of a heterologous promoter. - View Dependent Claims (30, 31, 32)
- -like cell to a subject, wherein the β
-
33. A method of treating diabetes in a subject comprising providing an effective amount of a composition comprising a β
- -like cell to a subject, wherein the β
-like cell comprises an expression cassette comprising a nucleic acid sequence encoding MafA under the control of a heterologous promoter. - View Dependent Claims (34, 35, 36, 37, 38)
- -like cell to a subject, wherein the β
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39. A composition comprising a β
- -like cell, wherein the β
-like cell comprises an expression cassette comprising a nucleic acid sequence encoding MafA under the control of a heterologous promoter. - View Dependent Claims (40, 41)
- -like cell, wherein the β
Specification