Analyte injection system
First Claim
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1. A method of applying a stacked analyte to a channel segment comprising:
- stacking one or more analytes in a channel;
detecting a voltage potential in the channel; and
, applying an electric field or a pressure differential along a channel segment when a selected voltage event is detected;
thereby applying the stacked analytes to the channel segment.
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Abstract
This invention provides methods and systems for injection of analytes into a separation channel for resolution and detection. Samples can be preconditioned and concentrated by isotachophoresis (ITP) before the injection is triggered by a detected voltage event. Separation of analytes from other sample constituents can be enhanced using skewing channel ITP.
128 Citations
86 Claims
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1. A method of applying a stacked analyte to a channel segment comprising:
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stacking one or more analytes in a channel;
detecting a voltage potential in the channel; and
,applying an electric field or a pressure differential along a channel segment when a selected voltage event is detected;
thereby applying the stacked analytes to the channel segment. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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35. A system for application of a stacked analyte to a channel segment comprising:
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a channel;
an analyte stacking in the channel; and
,a voltage detector in electrical contact with the channel, and in communication with a controller;
wherein the controller initiates a flow of electric current in a channel segment or a pressure differential along the channel segment when a selected voltage event is detected by the voltage detector. - View Dependent Claims (36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63)
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64. A method of separating an analyte of interest from a sample constituent not of interest, the method comprising:
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stacking the analyte by isotachophoresis in a channel comprising a skewing channel segment; and
,flowing the analyte and sample constituent not of interest through the skewing channel segment during or before the isotachophoresis, wherein the skewing channel segment comprises conditions providing a dispersion Peclet number more than 0.1 times a ratio of a skewing channel length over a skewing channel width. - View Dependent Claims (65, 66, 67, 68, 69, 70, 71, 72, 73, 74)
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75. A isotachophoresis system comprising:
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a channel comprising a skewing channel segment; and
,an analyte in the channel stacking by isotachophoresis;
wherein the skewing channel segment comprises conditions that provide a dispersion Peclet number more than 0.1 times a ratio of a skewing channel length over a skewing channel width. - View Dependent Claims (76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86)
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Specification