Generation of living tissue in vivo using a mold
First Claim
Patent Images
1. A method of making a living tissue construct having a predetermined shape, the method comprising obtaining a negative mold having a defined shape;
- suspending isolated tissue precursor cells in a hydrogel to form a liquid hydrogel-precursor cell composition;
filling the liquid hydrogel-precursor cell composition into the mold;
implanting the filled mold into a living host for a time sufficient to enable the tissue precursor cells to form tissue;
removing the mold from the living host; and
removing the living tissue construct from the mold.
2 Assignments
0 Petitions
Accused Products
Abstract
The invention features methods of making living tissue constructs having a predetermined shape by obtaining a negative mold having a defined shape; suspending isolated tissue precursor cells in a hydrogel to form a liquid hydrogel-precursor cell composition; filling the liquid hydrogel-precursor cell composition into the mold; implanting the filled mold into a living host for incubation; removing the mold after a suitable incubation period; and removing the living tissue construct from the mold. The final living tissue construct can then be surgically implanted into a patient.
-
Citations
21 Claims
-
1. A method of making a living tissue construct having a predetermined shape, the method comprising
obtaining a negative mold having a defined shape; -
suspending isolated tissue precursor cells in a hydrogel to form a liquid hydrogel-precursor cell composition;
filling the liquid hydrogel-precursor cell composition into the mold;
implanting the filled mold into a living host for a time sufficient to enable the tissue precursor cells to form tissue;
removing the mold from the living host; and
removing the living tissue construct from the mold. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 21)
-
-
15. A method of reconstructing an anatomical feature in a mammal, the method comprising
obtaining a suitable negative mold having a negative shape of the anatomical feature; -
suspending isolated tissue precursor cells in a hydrogel to form a liquid hydrogel-precursor cell composition, filling the liquid hydrogel-precursor cell composition into the mold;
inducing gel formation of the liquid hydrogel-precursor cell composition to form a living tissue construct;
implanting the filled mold into a living host for a time sufficient to enable the tissue precursor cells to form tissue;
removing the mold from the living host;
removing the tissue construct from the mold; and
implanting the tissue construct into the mammal.
-
Specification