Polynucleotide microarray including two or more groups of probe polynucleotides immobilized on substrate according to melting temperature and method for detecting target polynucleotides using the same

• US 20050123951A1
• Filed: 09/02/2004
• Published: 06/09/2005
• Est. Priority Date: 12/03/2003
• Status: Active Grant

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Paek, Sanghyun
• Granted Patent: US 7,338,764 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2527/107 Temperature of melting, i.e. Tm
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer

Abstract

Provided is a polynucleotide microarray including two or more groups of spots on which probe polynucleotides are immobilized, wherein the probe polynucleotides of each group have melting temperatures (Tms) within a predetermined range of Tm between the probe polynucleotides and target polynucleotides. A method for detecting target polynucleotides using the polynucleotide microarray is also provided.

Claims

1. A polynucleotide microarray comprising two or more groups of spots on which probe polynucleotides are immobilized, wherein the probe polynucleotides of each group have melting temperatures (Tms) within a predetermined range of Tm between the probe polynucleotides and target polynucleotides.

2. The polynucleotide microarray of claim 1, wherein a difference in Tm between the spots of each group is 10°

C. or less.

3. The polynucleotide microarray of claim 1, wherein a difference in Tm between the spots of each group is 5°

C. or less.

4. A method for detecting target polynucleotides, which comprises spotting samples containing the target polynucleotides onto the probe polynucleotides immobilized on the groups of the spots of the polynucleotide microarray of claim 1, followed by hybridization,

wherein at least one group of the spots is treated with a hybridization solution containing a denaturant, a stabilizer, or a mixture thereof, so that the deviation of Tms between the probe polynucleotides immobilized on the groups of the spots and the target polynucleotides is within a predetermined range.

5. The method of claim 4, wherein the target polynucleotides are labeled with a fluorescent marker and the method further comprises detecting the result of the hybridization using a fluorescent light.

6. The method of claim 4, wherein the denaturant is selected from the group consisting of formamide, urea, guanidine chloride, dimethylformamide (DMF), and a mixture thereof.

7. The method of claim 4, wherein the stabilizer is a salt selected from the group consisting of Na⁺, K⁺, Ca²⁺, and Mg²⁺.

8. The method of claim 4, wherein the deviation of the Tms is in a range of 0 to 5°

C.

9. The method of claim 4, wherein the deviation of the Tms is in a range of 0 to 3°

C.