Method for depleting specific nucleic acids from a mixture
First Claim
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1. A method for amplifying a nucleic acid sample from blood comprising:
- providing a nucleic acid sample from blood;
hybridizing at least one reduction oligonucleotide to at least one unwanted RNA in the sample;
incubating the mixture with an RNase H and subsequently inactivating the RNase H;
hybridizing a primer comprising oligo dT to the RNA in the mixture;
extending the primer to make cDNA; and
amplifying the cDNA.
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Abstract
The presently claimed invention provides methods, compositions, and apparatus for analyzing nucleic acids isolated from blood. Specifically, the present invention provides a method of analyzing blood samples by blocking amplification of selected unwanted RNAs and subsequently analyzing the amplified sample by hybridization to a plurality of probes attached to a solid support. In one embodiment, the invention provides enriching for a population of interest in a complex population by diminishing the presence of an unwanted sequence that may interfere with the analysis of sequences of interest.
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Citations
2 Claims
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1. A method for amplifying a nucleic acid sample from blood comprising:
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providing a nucleic acid sample from blood;
hybridizing at least one reduction oligonucleotide to at least one unwanted RNA in the sample;
incubating the mixture with an RNase H and subsequently inactivating the RNase H;
hybridizing a primer comprising oligo dT to the RNA in the mixture;
extending the primer to make cDNA; and
amplifying the cDNA.
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2-36. -36. (canceled)
Specification